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Combined enzymatic modification of stevioside and rebaudioside A

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Abstract

Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by Bacillus stearothermophilus B-5076 B. Macerans BIO-4m were the most effective biocatalysts. This method can be used successfully for direct transglycosylation of stevia extract without purification of its individual components.

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Authors and Affiliations

  1. Institute of Microbiology, National Academy of Science of Armenia, Abovyan, 375510, Armenia

    V. T. Kochikyan, L. A. Abelyan, A. M. Balayan & V. A. Abelyan

  2. Biotechnological Corporation Stevian, Kuala Lumpur, 50450, Malaysia

    A. A. Markosyan

Authors
  1. V. T. Kochikyan
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  2. A. A. Markosyan
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  3. L. A. Abelyan
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  4. A. M. Balayan
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  5. V. A. Abelyan
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Original Russian Text © V.T. Kochikyan, A.A. Markosyan, L.A. Abelyan, A.M. Balayan, V.A. Abelyan, 2006, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2006, Vol. 42, No. 1, pp. 37–43.

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Kochikyan, V.T., Markosyan, A.A., Abelyan, L.A. et al. Combined enzymatic modification of stevioside and rebaudioside A. Appl Biochem Microbiol 42, 31–37 (2006). https://doi.org/10.1134/S0003683806010030

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  • DOI: https://doi.org/10.1134/S0003683806010030

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