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Androgenesis in anther cultures of cultivars and a promising form of spring common wheat of West Siberia differing in the presence or absence of wheat-alien translocations

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Russian Journal of Genetics: Applied Research

Abstract

Androgenesis has been studied in the anther cultures of eight cultivars and one promising line of spring common wheat obtained in West Siberia (Siberian Research Institute of Agriculture, Omsk, Russia). The varieties are similar in origin but differ in the presence or absence of wheat-alien translocations (wheatrye 1RS.1BL and wheat-wheatgrass 7DL-7Ai). The promising form L-311/00-22 has the 1RS.1BL translocation and the cytoplasm of cultivated barley Hordeum vulgare L. The aim of this study was to assess whether it is possible to obtain dihaploid lines in the examined genotypes with wheat-alien translocations. It was found that different cultivars respond differently to anther-culture conditions depending on the concentration of 2,4-D in the initial medium. Form L-311/00-22 is best suited for androgenesis experiments and development of dihaploid lines. The effect of wheat-alien translocations depending on genetic background on androgenesis is discussed.

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References

  • Agache, S., Buyser, J., and Snape, J., Studies of the genetic relationship between anther culture and somatic tissue culture abilities in wheat, Plant Breed., 1988, vol. 100, pp. 26–33.

    Article  Google Scholar 

  • Agache, S., Bacheller, B., Buyse, J., et al., Genetic control of anther culture response in wheat using aneuploid, chromosome substitution and translocation lines, Theor. Appl. Genet., 1989, vol. 77, pp. 7–11.

    Article  Google Scholar 

  • Badaeva, E.D., Badaev, N.S., Gill, B.S., and Filatenko, A.A., Intraspecific karyotype divergence in Triticum araraticum, Plant Syst. Evol., 1994, vol. 192, no. 1, pp. 117–145.

    Article  Google Scholar 

  • Barnabas, B., Szakacs, Ě., Karsai, I., and Bedö. Z, In vitro androgenesis of wheat: from fundamental to practical application, Euphytica, 2001, vol. 119, pp. 211–216.

    Article  Google Scholar 

  • Belan, I.A., Rosseeva, L.P., Trubacheeva, N.V., et al., Important Agronomic Traits of Omskaya 37 Lines of Spring Wheat Cultivar Carrying Wheat-Rye Translocation 1RS.1BL, Russian Journal of Genetics: Applied Research, 2011, Vol. 1, No. 3, pp. 241–247.

    Google Scholar 

  • Belan, I.A. Rosseev, V.M., et al., Using of alien genetic material in spring bread wheat breeding in western Siberia, Eur. Cereals Genet. Co-op. Newslett., 2012, pp. 113–115.

    Google Scholar 

  • Belan, I.A., Rosseeva, L.P., Rosseev, V.M., et al., The study of economically valuable and adaptive traits in the line of the spring wheat variety Omsk 37 carrying translocations 1RS.1BL and 7DL-7Ai, Vavilov. Journal Genet. Breeding., 2012, vol. 16, no. 1, pp. 178–186.

    Google Scholar 

  • Chuang, C.C., Ouyang, J.W., Chia, H., et al., A set of potato media for wheat anther culture, Proc. Symp. Plant Tissue Culture. Peking: Sci. Press, 1978, pp. 51–56.

    Google Scholar 

  • Dobrovol’skaya, O.B., Pershina, L.A., Kravtsova, L.A., et al., The effect of rye chromosomes on the characteristic features of androgenesis in wheat-rye substitution lines Triticum aestivum L. (cv. Saratovskaya 29)/Secale cereale L. (cv. Onokhoiskaya) and Triticale, Russ. J. Genet., 2001, vol. 37, no. 5, pp. 506–512.

    Article  Google Scholar 

  • Dobrovol’skaya, O.B., Pershina, L.A., Kravtsova, L.A., and Shchapova, A.I., Comparative effects of rye chromosomes 1R and 5R on androgenesis in cultured anthers of wheat-rye substitution lines as dependent on the line origin, Russ. J. Genet., 2003, vol. 39, no. 4, pp. 467–470.

    Article  Google Scholar 

  • Dunwell, J.M., Pollen, ovule and embryo culture as tools in plant breeding, in Plant Tissue Culture and its Agricultural Applications, Withes, L.A. and Alderson, P.G., Eds., London: Butterworths, 1986, pp. 375–404.

    Google Scholar 

  • Foroughi-Wehr, B. and Zeller, F.J., In vitro microspore reaction of different German wheat cultivar, Theor. Appl. Genet., 1990, vol. 79, pp. 79–80.

    Article  Google Scholar 

  • Friebe, B., Jiang, J., Raupp, W.J., et al., Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status, Euphytica, 1996, vol. 91, pp. 59–87.

    Article  Google Scholar 

  • Gamborg, O.L. and Eveleigh, D.E., Culture methods and detection of glucanases in suspension cultures of wheat and barley, Can. J. Biochem., 1968, vol. 46, pp. 417421.

    Article  Google Scholar 

  • Guzy-Wrŏbelska, J. and Szarenko, I., Molecular and agronomic evaluation of wheat doubled haploid lines obtained through maize pollination and anther culture methods, Plant Breed., 2003, vol. 122, pp. 305–313.

    Article  Google Scholar 

  • Henry, Y. and Buyser, J., Effect of the 1B/1R translocation on anther-culture ability in wheat, Plant Cell Rep., 1985, vol. 4, pp. 307–310.

    Article  Google Scholar 

  • Hu, D., Tang, Y., Yuan, Z., and Wang, J., The induction of pollen sporophytes of winter wheat and the development of the new variety “Jiughua No. 1”, Sci. Agric. Sin., 1983, vol. 1, pp. 29–35.

    Google Scholar 

  • Ivanov, G.I., Biotechnological aspects of the creation of a source material for breeding cereal crops, Extended Abstract of Doctoral (Biol.) Dissertation, Krasnodar, 2006.

    Google Scholar 

  • Ji, J., Zhang, A., Wang, Z., et al., A wheat-Thinopyrum ponticum-rye trigeneric germplasm line with resistance to powdery mildew and stripe rust, Euphytica, 2012, vol. 188, pp. 199–207.

    Article  CAS  Google Scholar 

  • Joshi, R.K. and Nayak, S., Gene pyramiding—a broad spectrum technique for developing durable stress resistance in crop, Biotechnol. Mol. Biol. Rev., 2010, vol. 5, pp. 51–60.

    CAS  Google Scholar 

  • Kasha, K.J. and Maluszynski, M., Production of doubled haploids in crop plants, in Doubled Haploid Production in Crop Plants, Maluszynski, M., Kasha, K.J., Forster, B.P., and Szarejko, I., Eds., Dordrecht: Kluver Acad. Publ., 2003, pp. 1–4.

    Chapter  Google Scholar 

  • Laikova, L.I., Belan, I.A., Badaeva, E.D., et al., Development and study of spring bread wheat variety Pamyati Maystrenko with introgression of genetic material from synthetic hexaploid Triticum timopheevii Zhuk. Aegilops tauschii Coss., Russ. J. Genet., 2013, vol. 49, no. 1, pp. 89–97.

    Article  CAS  Google Scholar 

  • Lakin, G.F., Biometriya (Biometry), Moscow: Vyssh. Shk., 1980.

    Google Scholar 

  • Maluszynski, M., Szarejko, I., Barriga, P., and Balcerzyk, A., Heterosis in crop mutant crosses and production of high yielding lines using doubled haploid systems, Euphytica, 2001, vol. 120, pp. 387–398.

    Article  Google Scholar 

  • Oettler, G., Agronomic performance of anther-derived haploid and single seed descent lines in crosses between primary and secondary winter triticale, Plant Breed., 2008, vol. 127, pp. 476–479.

    Article  Google Scholar 

  • Oleszczuk, S., Sowa, S., and Zimny, J., Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (× Triticosecale Wittmack) cv. bogo, Plant Cell Rep., 2004, vol. 22, pp. 885–893.

    Article  PubMed  CAS  Google Scholar 

  • Oleszczuk, S., Rabiza-Swider, J., Zimny, J., and Lukaszewski, A.J., Aneuploidy among androgenic progeny of hexaploid triticale (× Triticisecale Wittmack), Plant Cell Rep., 2011, vol. 30, pp. 575–586.

    Article  PubMed  CAS  Google Scholar 

  • Pauk, J., Puolimatka, M., Lokos, T., and Monostori, T., In vitro androgenesia of triticale in isolated microspore culture, Plant Cell Tissue Organ Cult., 2000, vol. 61, pp. 221–229.

    Article  CAS  Google Scholar 

  • Pershina, L.A., Numerova, O.M., Belova, L.I., et al., Characteristic features of androgenesis in interspecific and intergeneric hybrids of common wheat, Sib. Biol. Zh., 1993, pp. 3–8.

    Google Scholar 

  • Pershina, L.A., Numerova, O.M., Belova, L.I., and Devyatkina, E.P., Biotechnological and cytogenetic aspects of producing new wheat genotypes using hybrids, Euphytica, 1998, vol. 100, nos. 1/3, pp. 239–244.

    Article  Google Scholar 

  • Pershina, L.A., Belova, L.I., Devyatkina, E.P., et al., The effectiveness of obtaining haploid plants in anther culture and in remote crosses of cereals, Fiziol. Biokhim. Kult. Rast., 1999a, vol. 31, no. 3, pp. 196–202.

    Google Scholar 

  • Pershina, L.A., Numerova, O.M., Belova, L.I., et al., Restoration of fertility in backcross progeny of barleywheat hybrids Hordeum vulgare L. (2n = 14) Triticum aestivum L. (2n = 42) in relation to wheat genotypes involved in backcrosses, Russ. J. Genet., 1999b, vol. 35, no. 2, pp. 176–183.

    CAS  Google Scholar 

  • Rabinovich, S.V., Importance of wheat-rye translocations for breeding modern cultivar of Triticum aestivum L., Euphytica, 1998, vol. 100, pp. 323–340.

    Article  Google Scholar 

  • Sági, L. and Barnabas, B., Evidence for cytoplasmic control of in vitro microspore embryogenesis in the anther culture of wheat (Triticum aestivum L.), Theor. Appl. Genet., 1989, vol. 78, pp. 867–872.

    Article  Google Scholar 

  • Servin, B., Martin, O.C., Mezard, M., and Hospital, F., Toward a theory of marker-assisted pyramiding, Genetics, 2004, vol. 168, pp. 513–523.

    Article  PubMed  CAS  Google Scholar 

  • Sibikeeva, Yu.E. and Sibikeev, S.N., Genetic analysis of anther culture response in wheat carrying alien translocations, Theor. Appl. Genet., 1996, vol. 92, pp. 782–785.

    Article  Google Scholar 

  • Sibikeeva, Yu.E., Sibikeev, S.N., and Krupnov, V.A., The effect of Lr19-translocation on in vitro androgenesis and inheritance of leaf-rust resistance in DH3 lines and F2 hybrids of common wheat, Russ. J. Genet., 2004, vol. 40, no. 9, pp. 1003–1006.

    Article  CAS  Google Scholar 

  • Tersi, M., Xynias, I.N., Gouli-Vadinoudi, E., and Roupakias, D.G., Anther culture response of f1 durum bread wheat hybrids after colchicines, Plant Breed., 2006, vol. 125, pp. 457–460.

    Article  Google Scholar 

  • Torp, A.M., Hansen, A.L., and Andersen, S.B., Chromosomal regions associated with green plant regeneration in wheat (Triticum aestivum L.) anther culture, Euphytica, 2001, vol. 119, pp. 377–387.

    Article  CAS  Google Scholar 

  • Trubacheeva, N.V., Rosseeva, L.P., Belan, I.A., et al., Characteristics of common wheat cultivars of West Siberia carrying the wheat-rye 1RS.1BL translocation, Russ. J. Genet., 2011, vol. 47, no. 1, pp. 13–18.

    Article  CAS  Google Scholar 

  • Ye, G. and Smith, K.F., Marker-assisted gene pyramiding for inbred lines development: basic principles and practical guidelines, Intern. J. Plant Breed., 2008, vol. 2, no. 1, pp. 1–10.

    Article  Google Scholar 

  • Zhonosar’, M.V. Optimization of the technology for obtaining double haploids of common wheat differing in the photoperiodic sensitivity genes (Ppd) and the vernalization duration (Vrd), Extended Abstract of Cand. (Biol.) Dissertation, Odessa, 2009.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    L. A. Pershina & T. S. Osadchaya

  2. Novosibirsk National Research State University, Novosibirsk, Russia

    L. A. Pershina

  3. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    E. D. Badaeva

  4. Siberian Research Institute of Agriculture, Russian Academy of Agricultural Sciences, Omsk, Russia

    I. A. Belan & L. P. Rosseeva

Authors
  1. L. A. Pershina
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  2. T. S. Osadchaya
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  3. E. D. Badaeva
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  4. I. A. Belan
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  5. L. P. Rosseeva
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Correspondence to L. A. Pershina.

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Original Russian Text © L.A. Pershina, T.S. Osadchaya, E.D. Badaeva, I.A. Belan, L.P. Rosseeva, 2013, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2013, Vol. 17, No. 1, pp. 40–49.

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Pershina, L.A., Osadchaya, T.S., Badaeva, E.D. et al. Androgenesis in anther cultures of cultivars and a promising form of spring common wheat of West Siberia differing in the presence or absence of wheat-alien translocations. Russ J Genet Appl Res 3, 246–253 (2013). https://doi.org/10.1134/S2079059713040096

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  • DOI: https://doi.org/10.1134/S2079059713040096

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