Abstract
New types of organic-inorganic hybrid nanocomposites based on nanosized titanium oxide(IV) (TiO2, particle size <100 nm) and carbon nanotubes (CNT, outer diameter of 10–15 nm, inner diameter of 2–6 nm, and length of 0.1–10 μm) and phosphatidylcholine were elaborated for improvement of analytical characteristics of screen printed electrodes. These nanomaterials were employed as an interface for immobilization of skeletal myoglobin. Electroanalytical and electrokinetic behavior of myoglobin on such interfaces was characterized with cyclic voltammetry (CV) and square wave voltammetry (SWV). Direct unmediated electron transfer between heme of immobilized myoglobin and electrodes modified with titanium oxide or carbon nanotubes was registered. The midpoint (redox) potential of the myoglobin Fe3+/Fe2+ E 1/2 = −0.263 V for electrodes modified with CNT and E 1/2 = −0.468 V for electrodes modified with TiO2 was observed (vs. Ag/AgCl reference electrode).
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Shumyantseva, V.V., Suprun, E.V., Bulko, T.V., Dobrynina, O.V., and Archakov, A.I., Biomed. Khim., 2010, vol. 56, pp. 55–71.
Dalle-Donne, I., Scaloni, A., and Butterfield, D.A., in Redox Proteomics, Willey Interscience, 2006, pp. 651–667.
Hasanzadeh, M., Shadjou, N. Eskandani, M., and Omidinia, M., Trends Anal. Chem., 2013, vol. 49, pp. 20–30.
Lewis, D.F.V., Guide to Cytochrome P450. Structure and Function. London and New York: Taylor and Francis, 2001.
Archakov, A.I. and Bachmanova, G.I., Cytochrome P450 and Active Oxygen, London: Taylor and Francis, 1990.
Hu, C. and Hu, S., J. Sensors, 2009, article ID 187615, 1–40.
Zhang, W., Chen, M., Gong, X., and Diao, G., Carbon, 2013, vol. 61, pp. 154–163.
Jia, L., Lu, Y., Shao, J., Liang, X., and Xu, Y., Trends Biotechnol., 2013, vol. 31, pp. 99–107.
Justino, C., Rocha-Santos, T., and Duarte, A., Trends Anal. Chem., 2013, vol. 45, pp. 24–36.
Kotanena, C., Moussy, F., Carrara, S., and Guiseppi-Elie, A., Biosens. Bioelectron., 2012, vol. 35, pp. 14–26.
Howladera, M., Doyle, T., Mohtashami, S., and Kish, J., Sensors Actuators B, 2013, vol. 178, pp. 132–139.
Archakov, A.I., Ivanov, Y.D., Lisitsa, A.V., and Zgoda, V.G., Proteomics, 2009, vol. 9, pp. 1326–1343.
Xiao, Y. and Li, C., Electroanalysis, 2008, vol. 20, pp. 648–662.
Suprun, E., Bulko, T., Lisitsa, A., Gnedenko, O., Ivanov, A., Shumyantseva, V., and Archakov, A., Biosens. Bioelectron., 2010, vol. 25, pp. 1694–1698
Suprun, E., Saveliev, A., Evtugyn, G., Lisitsa, A., Bulko, T., Shumyantseva, V., and Archakov, A., Biosens. Bioelectron., 2012, vol. 15, pp. 158–164.
Kulys, J. and D’Costa, E.J., Biosens. Bioelectron., 1991, vol. 6, pp. 109–115.
Papyshev, I., Vestnik RGMU, 2010, no. 5, pp. 69–72.
McDonnell, B., McDonnell, S., Hearty, S., Leonard, P., and O’Kennedy, R., Clin. Biochem., 2009, vol. 42, pp. 549–561.
Sholtz, F., Elektroanaliticheskie metody. Teoriya i praktika (Electroanalytical methods. Theory and practice. Moscos: BIONOM. Laboratoriya znanii, 2006.
Fujishima, A. and Zhang, X., C. R. Chimie, 2006, vol. 9, pp. 750–760.
Li, Q., Luo, G., and Feng, J., Electroanalysis, 2001, vol. 13, pp. 359–363.
Kamat, P., J. Phys. Chem. C, 2012, vol. 116, pp. 11849–11851.
Sun, W., Guo, Y., Ju, X., Zhang, Y., Wang, X, and Sun, Z., Biosens. Bioelectron., 2013, vol. 42, pp. 207–213.
Kumar, A., Lo, P., and Chen, S., Nanotechnology, 2008, vol. 19, 255501, 1–7.
Bistolas, N., Wollenberger, U., Jung, C., and Scheller, F.W., Biosens Bioelectron., 2005, vol. 20, pp. 2408–2423.
Shumyantseva, V., Bulko, T., Suprun, E., Chalenko, Y., Vagin, M., Rudakov, Y., Shatskaya, M., and Archakov, A., Biochim. Biophys. Acta, (Proteins and Proteomics), 2011, vol. 1814, pp. 94–101.
Li, S., Wang, J., Wei, H., Yang, Y., Bu, D., Zhang, L., and Zhou, L., J. Integrative Agr., 2012, vol. 11, pp. 439–445.
Zeng, Y., Chen, H., Shiau, K., Hung, S., Wang, Y., and Wu, C., Proteomics, 2012, vol. 12, pp. 380–390.
He, X., Chen, Z., Wang, Y., Wang, K., Su, J., and Yan, G., Biosens. Bioelectron., 2012, vol. 35, pp. 134–139.
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Original Russian Text © V.V. Shumyantseva, T.V. Bulko, A.V. Kuzikov, R. Khan, A.I. Archakov, 2014, published in Biomeditsinskaya Khimiya.
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Shumyantseva, V.V., Bulko, T.V., Kuzikov, A.V. et al. Development of methods for functionalization of screen printed electrodes with biocompatible organic-inorganic hybrid nanocomposites for biosensing applications. Biochem. Moscow Suppl. Ser. B 8, 237–242 (2014). https://doi.org/10.1134/S1990750814030123
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DOI: https://doi.org/10.1134/S1990750814030123