Abstract
The study of enzymatic and protective properties of recombinant IgA1 protease in active and mutant form has shown that the active form of IgA1 protease exhibited species- and type-specificity for mouse and human immunoglobulins. A mutant form, lacking enzymatic activity, had protective properties against meningococcal infection, induced by meningococcus serogroup A, B and C; it protected mice from lethal infection by live virulent cultures of heterologous serogroups of meningococcus. The results obtained in this study suggest that IgA1 protease may be considered as a perspective preparation at the stages of development of a polyvalent vaccine for protection of human against meningococcal infections of various etiology.
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References
Platonov, A.E., Kharit, S.M., and Platonova, O.V., Epidemiologiya i Vaktsinoprofilaktika, 2009, vol. 48, no. 5, pp. 32–45.
Frasch, C.E., Meningococcal Vaccines, 1995, vol. 7, pp. 245–283.
Donnelly, J.J., Deck, R.R., and Liu, M.A., J. Immunol., 1990, vol. 145, pp. 3071–3079.
Sierra, G.V.G., Campa, H.C., Varacel, N.M., Garcia, I.L., Izquierdo, P.L., Sotolongo, P.F., Casanueva, G.V., Rico, C.O., Rodriguez, C.R., and Terry, M.H., NIPH Ann., 1981, vol. 14, pp. 195–210.
Mulks, M.H. and Plaut, A.G., N. Engl. J. Med., 1978, vol. 299, pp. 973–976.
Plaut, A.G., Annu. Rev. Microbiol., 1981, vol. 37, pp. 603–622.
McNabb, P.C. and Tomasi, T.B., Annu. Rev. Microbiol., 1981, vol. 35, pp. 477–496.
Mistry, D. and Stockley, R.A., Int. J. Biochem. Cell. Biol., 2006, vol. 38, pp. 1244–1248.
Kazeeva, T.N. and Sheveleva, A.B., Biochemistry (Moscow), 2007, vol. 72, pp. 603–614.
Yagudaeva, E.Yu., Zhigis, L.S., Zueva, V.S., Mel’nikov, E.E., Zubov, V.P., Kozlov, L.V., Bichucher, A.M., Kotel’nikova, O.V., Alliluev, A.P., and Rumsh, L.D., Bioorgan. Khim., 2010, vol. 36, pp. 96–105.
Serova, O.V., Mel’nikov, E.E., Zinchenko, A.A., Kotel’nikova, O.V., Alliluev, A.P., Bichucher, A.M., Gordeeva, E.A., Zhigis, L.S., Zueva, V.S., Kozlov, L.V., Melikhova, T.D., Nokel, E.A., Yagudaeva, E.Yu., and Rumsh, L.D., Biofarmatsevticheskii Zhurnal, 2012, vol. 3, no. 6, pp. 42–47.
Yagudaeva, E.Yu., Alliluev, A.P., Gordeeva, E.A., Zhigis, L.C., Zinchenko, A.A., Zueva, V.C., Kozlov, L.V., Kotel’nikova, O.V., Melikhova, T.D., Nokel, E.A., Razgulyaeva, O.A., Serova, O.V., Sitnikova, E.A., and Rumsh, L.D., Novye informatsionnye tehnologii v meditsine, farmakologii, biologii i ekologii (New Informational Technologies in Medicine, Pharmacology, Biology, and Ecology), Proc. XX Int. Conf., Yalta-Gursuf, 2012, pp. 101–102.
Gregory, J.L., Rundegren, J., and Arnold, R.R., J. Immunol. Meth., 1987, vol. 99, pp. 101–106.
Kozlov, L.V., Romanov, S.V., D’yakov, V.L., Surovtsev, V.I., and Teimurazov, M.G., Rus. Patent no. 2310853, Byul. no. 32, 20.11.2007.
Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.
Kotel’nikova, O.V., Chibiskova, O.V., Nesmeyanov, V.A., Alliluev, A.P., Vol’pina, O.M., Koroev, D.O., Zhmak, M.N., Titova, M.A., and Ivanov, V.T., Byul. Eksper. Biol. Med., 2005, no. 5, pp. 553–556.
Alliluev, A.P., Kotel’nikova, O.V., Kuvakina, V.I., Bobyleva, G.V., Gofman, I.L., and Kaz’mina, Yu.G., Zh. Mikrobiol. Epidemiol., Immunol., 1995, no. 4, pp. 67–71.
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Original Russian Text © O.V. Kotel’nikova, A.P. Alliluev, E.Yu. Drozhzhina, I.S. Koroleva, E.A. Sitnikova, A.A. Zinchenko, E.A. Gordeeva, T.D. Melikhova, E.A. Nokel, L.S. Zhigis, V.S. Zueva, O.A. Razgulyaeva, O.V. Serova, E.Yu. Yagudaeva, L.D. Rumsh, 2013, published in Biomeditsinskaya Khimiya.
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Kotel’nikova, O.V., Alliluev, A.P., Drozhzhina, E.Y. et al. Protective properties of recombinant IgA1 protease from meningococcus. Biochem. Moscow Suppl. Ser. B 7, 305–310 (2013). https://doi.org/10.1134/S1990750813040033
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DOI: https://doi.org/10.1134/S1990750813040033