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Comparison of Changes in the Content of Plasma Membrane and Tonoplast Sterols under Oxidative and Osmotic Stress

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract—

We compared oxidative and osmotic stress-induced changes in sterol content in plasma membranes and tonoplasts isolated from stored beet (Beta vulgaris L.) roots. The most significant differences between the membranes under all studied stresses were observed in the cholesterol content: it decreased in the plasmalemma, but increased 4-6 times in the tonoplast. Similar changes were observed for other sterols, but in different ways under different types of stress. Particularly noticeable differences were noted under hyperosmotic stress. The increase in the content of sterols was much more pronounced in the vacuolar membrane compared to the plasmalemma. This observation allows us to conclude that tonoplast plays a more significant role in protection of the plant cell from stress compared to plasmalemma.

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Funding

The work was partially supported by the Russian Foundation for Basic Research (project no. 19-04-00013). The equipment of the Center for Collective Use “Bioanalytics” of Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences (Irkutsk) was used.

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia

    N. V. Ozolina, V. V. Gurina, I. S. Kapustina, E. V. Spiridonova & V. N. Nurminsky

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  1. N. V. Ozolina
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  2. V. V. Gurina
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  3. I. S. Kapustina
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  4. E. V. Spiridonova
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  5. V. N. Nurminsky
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Correspondence to N. V. Ozolina.

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The authors declare that they have no conflict of interest.

This article does not contain any studies with human participants or animals performed by any of the authors.

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Translated by E. Makeeva

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Ozolina, N.V., Gurina, V.V., Kapustina, I.S. et al. Comparison of Changes in the Content of Plasma Membrane and Tonoplast Sterols under Oxidative and Osmotic Stress. Biochem. Moscow Suppl. Ser. A 17, 180–182 (2023). https://doi.org/10.1134/S1990747823020058

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  • DOI: https://doi.org/10.1134/S1990747823020058

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