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Regulation of the Structural Stability of Erythrocytes by Hydrogen Peroxide: Mathematical Model and Experiment

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

In this study, the regulatory mechanisms induced by extracellular hydrogen peroxide were analyzed on the basis of a mathematical model that considers the key stages of the formation of methemoglobin and ferrylhemoglobin, as well as their binding to the erythrocyte membrane. Numerical modeling has shown that reversible binding of methemoglobin to the membrane is an adaptive mechanism aimed at stabilizing the lipid bilayer of the membrane. On the other hand, an increase in the concentration of ferrylhemoglobin and its binding to the membrane leads to an increase in pathophysiological processes that reduce the structural stability of cells. The quantity of methemoglobins and ferrylhemoglobins formed depends on the concentration of extracellular hydrogen peroxide and exposition time, the number of cells in the sample, the state of the antioxidant system of erythrocytes, the metabolic activity of cells and external metabolic conditions. Based on numerical modeling, optimal conditions (oxidant concentration and exposition time) have been determined, under which the activation of adaptive processes occurs. Experiments with erythrocyte hemolysis in vitro have shown that hydrogen peroxide at concentrations of 10–200 μM causes an increase in the structural stability of the membrane and a decrease in the proportion of hemolyzed erythrocytes.

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ACKNOWLEDGMENTS

The work was partially supported by the Belarusian Republican Foundation for Basic Research (project no. B20U-1).

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Authors and Affiliations

  1. Belarusian State University, 220030, Minsk, Belarus

    V. V. Voinarouski & G. G. Martinovich

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  1. V. V. Voinarouski
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Correspondence to V. V. Voinarouski.

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The authors declare that they have no conflict of interest.

All procedures performed in studies involving human participants were in accordance with the ethical standards of the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by E. Puchkov

APPENDIX

APPENDIX

The system of differential equations used in this study is presented below. In this form, the system was presented in the Wolfram Mathematica software.

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Voinarouski, V.V., Martinovich, G.G. Regulation of the Structural Stability of Erythrocytes by Hydrogen Peroxide: Mathematical Model and Experiment. Biochem. Moscow Suppl. Ser. A 16, 91–105 (2022). https://doi.org/10.1134/S1990747822010093

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