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Influence of blue light on the structure stability of antenna complexes from Allochromatium minutissimum with different content of carotenoids

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Effects of photooxidation of bacteriochlorophyll (absorbtion at 850 nm) from the light-harvesting complex LH2 of Alc. minutissimum membranes on the LH2 complex structure have been studied. Photooxidation was induced by blue light that is absorbed by carotenoids. Four samples with different levels (from 100% to 3–5%) and composition of carotenoids were obtained by inhibiting the carotenoid biosynthesis in bacteria with diphenylamine. Electrophoresis in polyacrylamide gel showed that after illumination LH2 complex contained all the oxidized bacteriochlorophyll. The carotenoid composition did not change after the oxidation of the main part of bacteriochlorophyll in the LH2 complex. The results suggest that oxidation takes place in the bacteriochlorophyll part, which is essential for the molecule optical properties (the system of double conjugated bonds is changed), but does not influence the stability of the structure of the LH2 complex.

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Abbreviations

LH2 complexes:

peripheral light-harvesting complex with 800 and 850 nm absorbtion peaks

LH1:

pericentric light-harvesting complex with 890 nm absorbtion peak

RC:

reaction center

BChl850:

bacteriochlorophyll of light-harvesting LH2 complex with 850 nm absorbtion peak

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    Z. K. Makhneva, M. A. Bol’shakov, Yu. E. Erokhin & A. A. Moskalenko

  2. Orel State University, Orel, Russia

    A. A. Ashikhmin

Authors
  1. Z. K. Makhneva
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  2. M. A. Bol’shakov
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  3. A. A. Ashikhmin
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  4. Yu. E. Erokhin
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  5. A. A. Moskalenko
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Correspondence to A. A. Moskalenko.

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Original Russian Text © Z.K. Makhneva, M.A. Bol’shakov, A.A. Ashikhmin, Yu.E. Erokhin, A.A. Moskalenko, 2009, published in Biologicheskie Membrany, 2009, Vol. 26, No. 3, pp. 188–193.

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Makhneva, Z.K., Bol’shakov, M.A., Ashikhmin, A.A. et al. Influence of blue light on the structure stability of antenna complexes from Allochromatium minutissimum with different content of carotenoids. Biochem. Moscow Suppl. Ser. A 3, 123–127 (2009). https://doi.org/10.1134/S1990747809020032

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  • DOI: https://doi.org/10.1134/S1990747809020032

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