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Native electrophoresis in cell proteomic: BN-PAGE and CN-PAGE

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Abstract

The mini review is intended to draw attention from researchers in Russia to a fast, cheap, and easily reproducible method of native polyacrylamide gel electrophoresis (PAGE), which for some reason has not yet found demand in our country. The review distinguishes between three types of native electrophoresis (BN-PAGE, CN-PAGE, and hrCN-PAGE) to study the proteomes of various animal, plant, and bacterial cells. References on the fundamental reviews, basic protocols, modifications of the initial methods and the examples of the combination of native electrophoresis and other chemical or physical methods are presented. Particular attention is paid to the principles of BN-, CN-, and hrCN-PAGE, as well as to their advantages and disadvantages

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  1. Sechenov Institute for Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    S. A. Shukolyukov

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Original Russian Text © S.A. Shukolyukov, 2011, published in Tsitologiya, Vol. 53, No. 2, 2011, pp. 159–165.

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Shukolyukov, S.A. Native electrophoresis in cell proteomic: BN-PAGE and CN-PAGE. Cell Tiss. Biol. 5, 311–318 (2011). https://doi.org/10.1134/S1990519X11030126

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