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Realization pathways of prolactin modulating effect on the cAMP-dependent mechanism of meiosis regulation in bovine oocytes

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Abstract

Cyclic AMP is one of the key regulators of mammalian meiosis. In the present work, realization pathways of the previously revealed modulating effect of prolactin (PRL) on the cAMP-dependent mechanism of meiosis regulation in bovine oocytes were studied. A comparative investigation of individual and combined effects of PRL (50 ng/ml) and an activator of adenylate cyclase forskolin (FK, 20 µM) on the meiotic reinitiation and completion of nuclear maturation in cumulus-surrounded and cumulus-free oocytes was performed. It has been shown that the pattern of the effects of PRL on the meiotic resumption in oocytes devoid of cumulus cells depends on the presence of FK in the culture medium. Furthermore, the realization of this effect is not associated with the activation of cytoplasmic isoforms of protein kinase C. It has also been found that PRL inhibits the retarding action of FK on the completion of oocyte nuclear maturation both in the presence and absence of cumulus cells. These findings suggest that PRL may modulate the functioning of the cAMP-dependent mechanism of meiosis regulation by the direct action on bovine oocytes, with realization of this action being independent of the metabolic coupling of oocytes with cumulus cells.

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Abbreviations

DO:

denuded oocytes (oocytes free of cumulus cells)

Dp:

diplotene

MII:

metaphase II

OCC:

oocyte-cumulus complexes

PRL:

prolactin

TI:

telophase I

FK:

forskolin

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Authors and Affiliations

  1. All-Russia State Research Institute of Animal Breeding, Russian Academy of Agricultural Sciences, Podolsk, Russia

    I. Yu. Lebedeva, G. N. Singina & L. K. Ernst

  2. Department of Animal Genetics, Breeding and Biotechnology, St. Petersburg State Agrarian University, St. Petersburg-Pushkin, Russia

    A. K. Golubev

Authors
  1. I. Yu. Lebedeva
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  2. G. N. Singina
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  3. L. K. Ernst
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  4. A. K. Golubev
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Correspondence to I. Yu. Lebedeva.

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Original Russian Text © I.Yu. Lebedeva, G.N. Singina, L.K. Ernst, A.K. Golubev, 2009, published in Tsitologiya, Vol. 51, No. 7, 2009, pp. 559–564.

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Lebedeva, I.Y., Singina, G.N., Ernst, L.K. et al. Realization pathways of prolactin modulating effect on the cAMP-dependent mechanism of meiosis regulation in bovine oocytes. Cell Tiss. Biol. 3, 438–444 (2009). https://doi.org/10.1134/S1990519X0905006X

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