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Subpopulation Heterogeneity of NK Cells during the Genetic Modification for Subsequent Use in Targeted Therapy

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Obtaining genetically engineered NK cells is a developing area of immunotherapy. In this work, we analyzed the subset heterogeneity of NK cells subjected to retroviral transduction, taking into account the content of adaptive NK cell progenitors. It was shown that subsets KIR2DL2/DL3+, as well as CD57–KIR2DL2/DL3+NKG2C+, can be modified with greater efficiency than the corresponding subsets that do not carry the KIR2DL2/DL3 and NKG2C markers. After genetic modification, the CD57–KIR2DL2/DL3+NKG2C+ cells began to express CD57 de novo, acquiring the adaptive NK cell phenotype.

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Funding

The study was supported by the Ministry of Education and Science of the Russian Federation (project no. 075-15-2021-1049).

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    M. A. Streltsova, A. A. Boyko, M. O. Ustiuzhanina, A. I. Palamarchuk, N. A. Alekseeva, R. A. Velichinskii, J. D. Vavilova, M. V. Grechikhina, A. M. Sapozhnikov,  S. M. Deev & E. I. Kovalenko

  2. Skolkovo Institute of Science and Technology, Moscow, Russia

    M. O. Ustiuzhanina

  3. Sechenov First Moscow State Medical University, Ministry of Healthcare of the Russian Federation, 119992, Moscow, Russia

    S. M. Deev

Authors
  1. M. A. Streltsova
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  2. A. A. Boyko
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  3. M. O. Ustiuzhanina
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  4. A. I. Palamarchuk
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  5. N. A. Alekseeva
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  6. R. A. Velichinskii
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  7. J. D. Vavilova
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  8. M. V. Grechikhina
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  9. A. M. Sapozhnikov
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  10. S. M. Deev
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  11. E. I. Kovalenko
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Corresponding author

Correspondence to E. I. Kovalenko.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. Human studies were reviewed and approved by Pirogov Russian National Research Medical University. All participants provided oral informed consent to participate in this study.

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Translated by M. Batrukova

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Streltsova, M.A., Boyko, A.A., Ustiuzhanina, M.O. et al. Subpopulation Heterogeneity of NK Cells during the Genetic Modification for Subsequent Use in Targeted Therapy. Dokl Biochem Biophys 507, 380–382 (2022). https://doi.org/10.1134/S1607672922340142

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  • DOI: https://doi.org/10.1134/S1607672922340142

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