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Biotechnological Method of Preparation and Characterization of Recombinant Antimicrobial Peptide Avicin A from Enterococcus avium

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Avicin A is a bacteriocin from the gram-positive bacterium Enterococcus avium. It exhibits a high microbicidal activity against bacteria of the genus Listeria, a causative agent of the severe human infection listeriosis. We developed a biotechnological method for obtaining avicin A and characterized its structure and biological activity. We also proposed a possible mechanism of the antimicrobial action of avicin A.

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REFERENCES

  1. Cotter, P.D., Ross, R.P., and Hill, C., Bacteriocins—a viable alternative to antibiotics?, Nat. Rev. Microbiol., 2013, vol. 11, no. 2, pp. 95–105.

    Article  CAS  PubMed  Google Scholar 

  2. Birri, D.J., Brede, D.A., Forberg, T., Holo, H., and Nes, I.F., Molecular and genetic characterization of a novel bacteriocin locus in Enterococcus avium isolates from infants, Appl. Environ. Microbiol., 2010, vol. 76, no. 2, pp. 483–492.

    Article  CAS  PubMed  Google Scholar 

  3. Rios, ColomboN.S., Chalon, M.C., Navarro, S.A., and Bellomio, A., Pediocin-like bacteriocins: new perspectives on mechanism of action and immunity, Curr. Genet., 2018, vol. 64, no. 2, pp. 345–351.

    Article  CAS  Google Scholar 

  4. Shenkarev, Z.O., Panteleev, P.V., Balandin, S.V., Gizatullina, A.K., Altukhov, D.A., Finkina, E.I., Kokryakov, V.N., Arseniev, A.S., and Ovchinniko-va, T.V., Recombinant expression and solution structure of antimicrobial peptide aurelin from jellyfish Aurelia aurita, Biochem. Biophys. Res. Commun., 2012, vol. 429, nos. 1–2, pp. 63–69.

    Article  CAS  PubMed  Google Scholar 

  5. Gallagher, N.L., Sailer, M., Niemczura, W.P., Nakashima, T.T., Stiles, M.E., and Vederas, J.C., Three-dimensional structure of leucocin a in trifluoroethanol and dodecylphosphocholine micelles: Spatial location of residues critical for biological activity in type IIa bacteriocins from lactic acid bacteria, Biochemistry, 1997, vol. 36, no. 49, pp. 15062–15072.

    Article  CAS  Google Scholar 

  6. Uteng, M., Hauge, H.H., Markwick, P.R.L., Fimland, G., Mantzilas, D., Nissen-Meyer, J., and Muhle-Goll, C., Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge, Biochemistry, 2003, vol. 42, no. 39, pp. 11417–11426.

    Article  CAS  Google Scholar 

  7. Arbulu, S., Lohans, C.T., van Belkum, M.J., Cin-tas, L.M., Herranz, C., Vederas, J.C., and Hernán-dez, P.E., Solution structure of enterocin HF, an antilisterial bacteriocin produced by Enterococcus faecium M3K31, J. Agric. Food Chem., 2015, vol. 63, no. 49, pp. 10689–10695.

    Article  CAS  PubMed  Google Scholar 

  8. Minahk, C.J., Farías, M.E., Sesma, F., and More-ro, R.D., Effect of enterocin CRL35 on Listeria monocytogenes cell membrane, FEMS Microbiol. Lett., 2000, vol. 192, no. 1, pp. 79–83.

    Article  CAS  PubMed  Google Scholar 

  9. Kjos, M., Nes, I.F., and Diep, D.B., Class II one-peptide bacteriocins target a phylogenetically defined subgroup of mannose phosphotransferase systems on sensitive cells, Microbiology, 2009, vol. 155, pp. 2949–2961.

    Article  CAS  Google Scholar 

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Correspondence to S. V. Balandin.

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Translated by M. Batrukova

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Balandin, S.V., Finkina, E.I., Nurmukhamedova, E.KA. et al. Biotechnological Method of Preparation and Characterization of Recombinant Antimicrobial Peptide Avicin A from Enterococcus avium. Dokl Biochem Biophys 484, 42–44 (2019). https://doi.org/10.1134/S1607672919010113

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  • DOI: https://doi.org/10.1134/S1607672919010113

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