Skip to main content
SpringerLink
Log in

Effeсts of the 70th Amino Acid Residue on the Photostability of FAST Complexes

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

We report a novel mutant of FAST–FAST T70V. The fluorescent properties of the complex were studied to show that the T70V mutation only slightly affects the fluorescent properties but has no effect on the photostability of the protein–fluorogen complex. Thus, evidence was obtained showing that the threonine alcohol group is not involved in the irreversible photodegradation of the complex, despite the possible contact of the amino acid with the fluorogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1.
Fig. 1.
Fig. 2.

REFERENCES

  1. Rodriguez, E.A., Campbell, R.E., Lin, J.Y., Lin, M.Z., Miyawaki, A., Palmer, A.E., Shu, X., Zhang, J., and Tsien, R.Y., Trends Biochem. Sci., 2017, vol. 42, pp. 111–129. https://doi.org/10.1016/j.tibs.2016.09.010

    Article  CAS  PubMed  Google Scholar 

  2. Chudakov, D.M., Matz, M.V., Lukyanov, S., and Lukyanov, K.A., Physiol. Rev., 2010, vol. 90, pp. 1103–63. https://doi.org/10.1152/physrev.00038.2009

    Article  CAS  PubMed  Google Scholar 

  3. Xu, S. and Hu, H.Y., Acta Pharm. Sin. B, 2018, vol. 8, pp. 339–348. https://doi.org/10.1016/j.apsb.2018.02.001

    Article  PubMed  PubMed Central  Google Scholar 

  4. Gallo, E., Bioconjug. Chem., 2020, vol. 31, pp. 16–27. https://doi.org/10.1021/acs.bioconjchem.9b00710

    Article  CAS  PubMed  Google Scholar 

  5. Mineev, K.S., Goncharuk, S.A., Goncharuk, M.V., Povarova, N.V., Sokolov, A.I., Baleeva, N.S., Smirnov, A.Y., Myasnyanko, I.N., Ruchkin, D.A., Bukhdruker, S., Remeeva, A., Mishin, A., Borshchevskiy, V., Gordeliy, V., Arseniev, A.S., Gorbachev, D.A., Gavrikov, A.S., Mishin, A.S., and Baranov, M.S., Chem. Sci., 2021, vol. 12, pp. 6719–6725. https://doi.org/10.1039/d1sc01454d

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Plamont, M.A., Billon-Denis, R., Maurin, S., Gauron, C., Pimenta, F.M., Specht, C.G., Shi, J., Quérard, J., Pan, B., Rossignol, J., Moncoq, K., Morellet, N., Volovitch, M., Lescop, E., Chen, Y., Triller, A., Vriz, S., Saux, T., Jullien, L., and Gautier, A., Proc. Natl. Acad. Sci., 2016, vol. 113, pp. 497–502. https://doi.org/10.1073/pnas.1513094113

    Article  CAS  PubMed  Google Scholar 

  7. Péresse, T. and Gautier, A., Int. J. Mol. Sci., 2019, vol. 20, pp. 6142. https://doi.org/10.3390/ijms20246142

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Li, C., Plamont, M.A., Sladitschek, H.L., Rodrigues, V., Aujard, I., Neveu, P., Saux, T., Jullien, L., and Gautier, A., Chem. Sci., 2017, vol. 8, pp. 5598–5605. https://doi.org/10.1039/C7SC01364G

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Povarova, N.V., Zaitseva, S.O., Baleeva, N.S., Smirnov, A.Y., Myasnyanko, I.N., Zagudaylova, M.B., Bozhanova, N.G., Gorbachev, D.A., Malyshevskaya, K.K., Gavrikov, A.S., Mishin, A.S., and Baranov, M.S., Chem. Eur. J., 2019, vol. 25, pp. 9592–9596. https://doi.org/10.1002/chem.201901151

    Article  CAS  PubMed  Google Scholar 

  10. Myasnyanko, I.N., Gavrikov, A.S., Zaitseva, S.O., Smirnov, A.Y., Zaitseva, E.R., Sokolov, A.I., Malyshevskaya, K.K., Baleeva, N.S., Mishin, A.S., and Baranov, M.S., Chem. Eur. J., 2021, vol. 27, pp. 3986–3990. https://doi.org/10.1002/chem.202004760

    Article  CAS  PubMed  Google Scholar 

  11. Ermakova, Y.G., Sen, T., Bogdanova, Y.A., Smirnov, A.Y., Baleeva, N.S., Krylov, A.I., Baranov, M.S., J. Phys. Chem. Lett., 2018, vol. 9, pp. 1958–1963. https://doi.org/10.1021/acs.jpclett.8b00512

    Article  CAS  PubMed  Google Scholar 

  12. Engler, C., Gruetzner, R., Kandzia, R., and Marillonnet, S., PLoS One, 2009, vol. 4, p. e5553. https://doi.org/10.1371/journal.pone.0005553

  13. Engler, C., Kandzia, R., and Marillonnet, S., PLoS One, 2008, vol. 3, p. e3647. https://doi.org/10.1371/journal.pone.0003647

  14. Weber, E., Engler, C., Gruetzner, R., Werner, S., and Marillonnet, S., PLoS One, 2011, vol. 6, p. e16765. https://doi.org/10.1371/journal.pone.0016765

  15. Goncharuk, M.V., Baleeva, N.S., Nolde, D.E., Gavrikov, A.S., Mishin, A.V., Mishin, A.S., Sosorev, A.Y., Arseniev, A.S., Goncharuk, S.A., Borshchevskiy, V.I., Efremov, R.G., Mineev, K.S., and Baranov, M.S., Comm. Biol., vol. 5, pp. 706. https://doi.org/10.1038/s42003-022-03662-9

Download references

Funding

The work was financially supported by the Russian Science Foundation (project no. 23-14-00042).

Author information

Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    Yu. A. Bogdanova, K. S. Mineev, N. S. Baleeva & M. S. Baranov

  2. Pirogov Russian National Research Medical University, 117997, Moscow, Russia

    M. S. Baranov

  3. Department of Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    M. S. Baranov

Authors
  1. Yu. A. Bogdanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. S. Mineev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. S. Baleeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. S. Baranov
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to the writing of the article.

Corresponding author

Correspondence to N. S. Baleeva.

Ethics declarations

The data that support the findings of this study are available from the corresponding author upon reasonable request. This article does not contain any studies conducted by any authors of this work. This article does not contain any studies involving patients or animals as test objects. Informed consent was not required for this article. No conflict of interest was declared by the authors.

Additional information

Publisher's Note. Pleiades Publishing remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bogdanova, Y.A., Mineev, K.S., Baleeva, N.S. et al. Effeсts of the 70th Amino Acid Residue on the Photostability of FAST Complexes. Russ J Bioorg Chem 49, 1489–1493 (2023). https://doi.org/10.1134/S1068162023060286

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162023060286

Keywords:

Search

Navigation