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Synthesis of tritium- and deuterium-labeled isopentenyladenine

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Abstract

The effect of catalysts and temperature on the solid-phase isotope exchange of isopentenyladenine with deuterium and tritium was studied. In the temperature interval 150–170°C, the reaction can be performed selectively, preserving the double bound of the initial compound. The formation of isotopomers was recorded by mass spectrometry. Slightly more than two deuterium atoms are incorporated, on the average, into isopentenyladenine molecule. The efficiency of the isotope exchange increases at the moment of hydrogenation of the initial compound. Deuterium- and tritium-labeled isopentenyladenine and dihydroisopentenyladenine were synthesized. Labeled isopentenyladenine is capable of specific binding with AHK4 cytokinin receptor.

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akad. Kurchatova 2, Moscow, 123182, Russia

    G. V. Sidorov & N. F. Myasoedov

  2. Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    S. N. Lomin & G. A. Romanov

Authors
  1. G. V. Sidorov
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  2. N. F. Myasoedov
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  3. S. N. Lomin
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  4. G. A. Romanov
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Corresponding author

Correspondence to G. V. Sidorov.

Additional information

Original Russian Text © G.V. Sidorov, N.F. Myasoedov, S.N. Lomin, G.A. Romanov, 2015, published in Radiokhimiya, 2015, Vol. 57, No. 1, pp. 94–96.

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Sidorov, G.V., Myasoedov, N.F., Lomin, S.N. et al. Synthesis of tritium- and deuterium-labeled isopentenyladenine. Radiochemistry 57, 108–110 (2015). https://doi.org/10.1134/S1066362215010178

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  • DOI: https://doi.org/10.1134/S1066362215010178

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