Abstract
Actinomycetic complexes of soils and plant substrates from tundra and taiga zones of Russia and Finland have been studied. A molecular cytogenetic technique—fluorescence in situ hybridization (FISH) method—has been applied to estimate the metabolically active component in the representatives of the Actinobacteria. In the prokaryotic microbial communities of studied plant substrates, the biomass of metabolically active psychrotolerant representatives of Actinobacteria was determined upon incubation at 5°C reached 34% of the total bacterial biomass; at the incubation temperature of 20°C, it increased to 56%. Under conditions of low temperature, psychrotolerant actinomycetes displayed active growth with the development of both substrate and aerial mycelium. The abundance of psychrotolerant actinomycetes reached thousands and tens of thousands CFU/g for taiga mosses in Finland and increased to millions CFU/g for tundra flowering plants in the Taimyr Peninsula of Russia. It decreased in the sequence: peat soil > plants > moss cushions > litter horizons. The length of actinomycetic mycelium in tundra plants varied from 98 m/g at 5°C to 291 m/g at 20°C; in taiga mosses, it varied from 120 to 180 m/g, respectively. The complex of psychrotolerant actinomycetes was mainly composed of colorless species of the Streptomyces genus possessing specific ecophysiological features, such as the change in the color of substrate and aerial mycelium, the appearance of pigmentation, and the increase in the growth rate upon higher incubation temperatures. More than 60% of actinomycetes isolated from taiga mosses of Finland were represented by psychrotolerant species of Micromonospora genus.
Similar content being viewed by others
REFERENCES
G. F. Gauze, T. P. Preobrazhenskaya, M. A. Sveshnikova, L. P. Terekhova, and T. S. Maksimova, Guide for Identification of Actinomycetes (Nauka, Moscow, 1983), p. 244.
N. A. Manucharova, A. N. Vlasenko, E. V. Men’ko, and D. G. Zvyagintsev, “Specificity of the chitinolytic microbial complex of soils incubated at different temperatures,” Microbiology (Moscow) 80, 205–215 (2011).
N. A. Manucharova, Yu. V. Kuteinikova, P. V. Ivanov, S. K. Nikolaeva, V. T. Trofimov, P. Yu. Stepanov, E. V. Tyapkina, D. N. Lipatov, and A. L. Stepanov, “Molecular analysis of the hydrolytic component of petroleum-contaminated soils and of soils remediated with chitin,” Microbiology (Moscow) 86, 395–402 (2017). https://doi.org/10.1134/S0026261717030092
Methods of Soil Microbiology and Biochemistry (Moscow State Univ., Moscow, 1991) [in Russian].
R. I. Amann, W. Ludwig, and K.-H. Schleifer, “Phylogenetic identification and in situ detection of individual microbial cells without cultivation,” Microbiol. Rev. 59, 143–169 (1995).
A. A. Bhatti, S. Haq, and R. A. Bhat, “Actinomycetes benefaction role in soil and plant health,” Microb. Pathog. 111, 458–467 (2017).
S. Fendrihan and T. Negoita, “Psychrophilic microorganisms as important source for biotechnological processes,” in Adaptation of Microbial Life to Environmental Extremes, Ed. by H. Stan-Lotter and S. Fendrihan (Springer-Verlag, New York, 2012), pp. 133–158.
V. Gesheva, “Production of antibiotics and enzymes by soil microorganisms from the windmill islands region, Wilkes Land, East Antarctica,” Polar Biol. 33 (10), 1351–1357 (2010).
V. Gesheva and T. Negoita, “Psychrotrophic microorganism communities of Haswell Island, Antarctica, and their biosynthetic potential,” Polar Biol. 35 (2), 291–297 (2012).
M. K. Männistö and M. M. Häggblom, “Characterization of psychrotolerant heterotrophic bacteria from Finnish Lapland,” Syst. Appl. Microbiol. 29 (3), 229–243 (2006).
M. K. Männistö, M. Tiirola, and M. M. Häggblom, “Bacterial communities in Arctic fjelds of Finnish Lapland are stable but highly pH-dependent,” FEMS Microbiol. Ecol. 59 (2), 452–465 (2007).
R. M. Nissinen, M. K. Männistö, and J. D. van Elsas, “Endophytic bacterial communities in three arctic plants from low arctic fell tundra are cold-adapted and host-plant specific,” FEMS Microbiol. Ecol. 82 (2), 510–522 (2012).
A. Raja, P. Prabakaran, and P. Gajalakshmi, “Isolation and screening of antibiotic producing psychrophilic actinomycetes and its nature from Rothan Hill soil against Streptococcus sp.,” Res. J. Microbiol. 5 (1), 44–49 (2010).
L. Selbmann, L. Zucconi, S. Ruisi, M. Grube, M. Cardinale, and S. Onofri, “Culturable bacteria associated with Antarctic lichens: affiliation and psychrotolerance,” Polar Biol. 33, 71–83 (2010).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by T. Chicheva
Rights and permissions
About this article
Cite this article
Prokopenko, V.V., Zenova, G.M. & Manucharova, N.A. Ecophysiological Characteristics of Psychrotolerant Actinomycetes in Tundra and Forest Landscapes. Eurasian Soil Sc. 52, 682–689 (2019). https://doi.org/10.1134/S1064229319040100
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1064229319040100