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Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site

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Abstract

Crystals of porcine pancreatic carboxypeptidase B (CPB) were grown by the capillary counter-diffusion method in the presence of polyethylene glycol and zinc acetate. The three-dimensional structure of CPB was determined at 1.40 Å resolution using the X-ray diffraction data set collected from the crystals of the enzyme at the SPring 8 synchrotron facility and was refined to R fact = 17.19%, R free = 19.78%. The structure contains five zinc atoms, two of which are present in the active site of the enzyme, and an acetate ion. The arrangement of an additional zinc atom in the active site and the acetate ion is different from that reported by Yoshimoto et al.

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    V. Kh. Akparov

  2. Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow, 119333, Russia

    V. I. Timofeev & I. P. Kuranova

  3. National Research Centre “Kurchatov Institute”, Moscow, 123098, Russia

    V. I. Timofeev & I. P. Kuranova

  4. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    N. N. Maghsoudi

Authors
  1. V. Kh. Akparov
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  2. V. I. Timofeev
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  3. N. N. Maghsoudi
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  4. I. P. Kuranova
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Corresponding author

Correspondence to V. Kh. Akparov.

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Original Russian Text © V.Kh. Akparov, V.I. Timofeev, N.N. Maghsoudi, I.P. Kuranova, 2017, published in Kristallografiya, 2017, Vol. 62, No. 2, pp. 237–242.

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Akparov, V.K., Timofeev, V.I., Maghsoudi, N.N. et al. Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site. Crystallogr. Rep. 62, 249–253 (2017). https://doi.org/10.1134/S106377451702002X

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  • DOI: https://doi.org/10.1134/S106377451702002X

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