Skip to main content
SpringerLink
Log in

Changes in the level of lectins in the mantle of the mussel Mytilus trossulus in response to anthropogenic contaminants

  • Biochemistry
  • Published:
Russian Journal of Marine Biology Aims and scope Submit manuscript

Abstract

The lectin activity in an extract from the mantle of the mussel Mytilus trossulus was tested for the first time. Using the method of the hemagglutination inhibition assay, it was shown that lectins were Gal/GalNAc-specific and best agglutinated with rabbit erythrocytes. The influence of foreign compounds on the lectin level in the M. trossulus mantle was examined. Upon cadmium acetate exposure, the level of lectin activity exhibited phasic alterations and depended on the contaminant concentration or the time of exposure. During exposure of mussels to a synthetic detergent or diesel fuel, changes in lectin contents were dependent on the time of exposure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Burlakova, E.B., Kondratov, A.A., and Maltseva, E.L., Effect of Minute Doses of Biologically Active Substances and Low-Intensity Physical Factors, Khim. Phizika, 2003, vol. 22, no. 2, pp. 21–40.

    CAS  Google Scholar 

  2. Galaktionov, V.G., Ocherki evolutsionnoi immunologii (Essays on Evolutionary Immunology), Moscow: Nauka, 1995.

    Google Scholar 

  3. Isaeva, V.V., Using of Molluscan Hemocytes and Echinoderm Coelomites for Biotesting, Biol. Morya, 1995, vol. 21, no. 6, pp. 378–385.

    Google Scholar 

  4. Loenko, Yu.N., Glazkova, V.E., Artyukov, A.A., and Ovodova, R.G., Lectins of Marine Invertebrates, Uspekhi sovrem. biol., 1992, vol. 112, no. 5–6, pp. 785–794.

    CAS  Google Scholar 

  5. Luk’yanov, P.A., Chernikov, O.V., Kobelev, S.S. et al., Carbohydrate-Binding Proteins of Marine Invertebrates, Bioorgan. Khimiya, 2007, vol. 33, no. 1, pp. 172–181.

    Google Scholar 

  6. Moore, J.W. and Ramamoorthy, S. (1987). Heavy metals in natural waters. Springer-Verlag, New York.

    Google Scholar 

  7. Polevshchikov, A.V., Lectins in Protective Reactions of Invertebrates, Zhurn. obshch. biol., 1996, vol. 57, pp. 718–739.

    Google Scholar 

  8. Suponina, A.P., Koryakova, M.D., and Gordinko, P.S., Chemical-Ecological Evaluation of Contamination of Coastal Sea Waters with Heavy Metals, In: Fiziko-khim. poverkhnostnaya zashchita materiala (Physical-Chemical Surface Protection of Materials), 2008, vol. 44, no. 5, pp. 525–529.

    Google Scholar 

  9. Chelomin, V.P., Belcheva, N.N., and Zakhartsev, M.V., Biochemical Mechanisms of Adaptation in the Mussel Mytilus trossulus to Ions of Cadmium and Copper, Biol. Morya, 1998, vol. 24, no. 5, pp. 319–325.

    CAS  Google Scholar 

  10. Yakovleva, N.V., Samoilovitch, M.P., and Gorbushin, A.M., Polyvalence of the Strategies of Defense of Mollusks Against Pathogens, Zhurn. evol. biokhim. i fiziol., 2001, vol. 37, no. 4, pp. 270–277.

    Google Scholar 

  11. Yaroslavtseva, L.M. and Sergeeva, E.P., Influence of Copper Ions on the Early Stages of Development in the Pacific Mussel Mytilus trossulus (Bivalvia), Biol. Morya, 2005, vol. 31, no. 4, pp. 267–273.

    Google Scholar 

  12. Al-Subiai, S.N., Jha, A.N., and Moody A.J., Contamination of Bivalve Haemolymph Samples by Adductor Muscle Components: Implications for Biomarker Studies, Ecotoxicology, 2009, vol. 18, no. 3, pp. 334–342.

    Article  CAS  PubMed  Google Scholar 

  13. Adhya, M., Singha, B., and Chatterjee, B.P., Purification and Characterization of an N-acetylglucosamine Specific Lectin from Marine Bivalve Macoma birmanica, Fish Shellf. Immunol., 2009, vol. 27, no. 1, pp. 1–8.

    Article  CAS  Google Scholar 

  14. Belogortseva, N.I., Molchanova, V.I., Kurika, A.V., and Glazkova, V.E., Isolation and Characterization of New GalNAc/Gal-Specific Lectin from the Sea Mollusk Crenomytilus grayanus, Comp. Biochem. Physiol. Part C, 1998, vol. 119, pp. 45–50.

    CAS  Google Scholar 

  15. Bulgakov, A.A., Park, K.-Il., and Choi, K.-S. et al., Purification and Characterisation of a Lectin Isolated from the Manila Clam Ruditapes philippinarum in Korea, Fish Shellf. Immunol., 2004, vol. 16, no. 4, pp. 487–499.

    Article  CAS  Google Scholar 

  16. Coles, J.A., Farley, S.R., and Pipe, R.K., Alteration of the Immune Response of the Common Marine Mussel Mytilus edulis Resulting from Exposure to Cadmium, Dis. Aquat. Org., 1995, vol. 22, pp. 59–65.

    Article  CAS  Google Scholar 

  17. Dyrynda, E.A., Dyrynda, P.E.J., and Law R.J. et al., Changes in Immune Parameters of Natural Mussel Mytilus edulis Populations Following a Major Oil Spill (’sea Empress’, Wales, UK), Mar. Ecol. Prog. Ser., 2000, vol. 206, no. 3, pp. 155–170.

    Article  Google Scholar 

  18. Fitzpatrick, J.L., Nadella, S., and Bucking, C. et al., The Relative Sensitivity of Sperm, Eggs and Embryos to Copper in the Blue Mussel (Mytilus trossulus), Comp. Biochem. Physiol. Part C, 2008, vol. 147, no. 4, pp. 441–449.

    CAS  Google Scholar 

  19. Gestal, C., Roch, P., and Renault, T. et al., Study of Diseases and the Immune System of Bivalves Using Molecular Biology and Genomics, Rev. Fish. Sci., 2008, vol. 16, no. S1, pp. 133–156.

    Article  CAS  Google Scholar 

  20. Iwanaga, S. and Lee, B.L., Recent Advances in Innate Immunity of Invertebrate Animals, J. Biochem. Mol. Biol., 2005, vol. 38, no. 2, pp. 128–150.

    CAS  PubMed  Google Scholar 

  21. Joline, R.W. and Leah, I.B.-Y., Influence of Food Quality and Salinity on Dietary Cadmium Availability in Mytilus trossulus, Aquat. Toxicol., 2007, vol. 81, no. 2, pp. 144–151.

    Article  Google Scholar 

  22. Kamiya, H., Possible Multiple Functions of the Invertebrate Humoral Lectins, Fish Pathol., 1995, vol. 30, no. 2, pp. 129–139.

    Google Scholar 

  23. Lares, M.L. and Orians, K.J., Differences in Cd Elimination from Mytilus californianus and Mytilus trossulus Soft Tissues, Environ. Pollut. 2001, vol. 112, no. 2, pp. 201–207.

    Article  CAS  PubMed  Google Scholar 

  24. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.L., Protein Measurements with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  PubMed  Google Scholar 

  25. Luengen, A.C., Friedman, C.S., Raimondi, P.T., and Flegal, A.R., Evaluation of Mussel Immune Responses as Indicators of Contamination in San Francisco Bay, Mar. Pollut. Bull., 2003, vol. 46, no. 8, pp. 954–958.

    Article  Google Scholar 

  26. Naganuma, T., Ogawa, T., and Hirabayashi, J. et al., Isolation, Characterization and Molecular Evolution of a Novel Pearl Shell Lectin from a Marine Bivalve, Pteria penguin, Mol. Div., 2006, vol. 10, no. 4, pp. 607–618.

    Article  CAS  Google Scholar 

  27. Park, J. and Kim, H., Isolation and Characterization of a Lectin from the Mussel, Mytilus edulis, Korean Biochem. J., 1987, vol. 20, no. 3, pp. 208–214.

    CAS  Google Scholar 

  28. Pempkowiak, J., Pazdro, K., and Kopecka, J. et al. Toxicants Accumulation Rates and Effects in Mytilus trossulus and Nereis diversicolor exposed separately or together to Cadmium and PAHs, J. Environ. Sci. Health. Tox. Hazard. Subst. Environ. Eng., 2006, vol. 41, no. 11, pp. 2571–2586.

    CAS  Google Scholar 

  29. Pipe, R.K., Coles, J.A., Carissan, F.M.M., and Ramanathan, K., Copper Induced Immunomodulation in the Marine Mussel, Mytilus edulis, Aquat. Toxicol., 1999, vol. 46, no. 1, pp. 43–54.

    Article  CAS  Google Scholar 

  30. Rainbow P.S., Wolowicz, M., and Fialkowski, W. et al., Biomonitoring of Trace Metals in the Gulf of Gdansk, Using Mussels (Mytilus trossulus) and Barnacles (Balanus improvisus), Water Res., 2000, vol. 34, no. 6, pp. 1823–1829.

    Article  CAS  Google Scholar 

  31. Sun, J., Wang, L., and Wang, B. et al., Purification and Characterisation of a Natural Lectin from the Serum of the Shrimp Litopenaeus vannamei, Fish Shellf. Immunol., 2007, vol. 23, no. 2, pp. 292–299.

    Article  CAS  Google Scholar 

  32. Szefer, P., Frelek, K., and Szefer, K. et al., Distribution and Relationships of Trace Metals in Soft Tissue, Byssus and Shells of Mytilus edulis trossulus from the Southern Baltic, Environ. Pollut., 2002, vol. 120, no. 2, pp. 423–444.

    Article  CAS  PubMed  Google Scholar 

  33. Tasumi, S. and Vasta, G.R., A Galectin of Unique Domain Organization from Hemocytes of the Sastern Oyster (Crassostrea virginica) is a Receptor for the Protistan Parasite Perkinsus marinus, J. Immunol., 2007, vol. 179, pp. 3086–3098.

    CAS  PubMed  Google Scholar 

  34. Wootton, E.C., Dyrynda, E.A., and Ratcliffe, N.A., Bivalve Immunity: Comparisons Between the Marine Mussel (Mytilus edulis), the Edible Cockle (Cerastoderma edule) and the Razor-Shell (Ensis siliqua), Fish Shellf. Immunol., 2003, vol. 15, pp. 195–210.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia

    I. V. Chikalovets, O. V. Chernikov, V. I. Molchanova & P. A. Lukyanov

  2. Far Eastern State University, Vladivostok, 690950, Russia

    E. A. Shekhova

Authors
  1. I. V. Chikalovets
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. V. Chernikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Shekhova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. I. Molchanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. A. Lukyanov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Chikalovets.

Additional information

Original Russian Text © I.V. Chikalovets, O.V. Chernikov, E.A. Shekhova, V.I. Molchanova P.A. Lukyanov, 2010, published in Biologiya Morya.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chikalovets, I.V., Chernikov, O.V., Shekhova, E.A. et al. Changes in the level of lectins in the mantle of the mussel Mytilus trossulus in response to anthropogenic contaminants. Russ J Mar Biol 36, 70–74 (2010). https://doi.org/10.1134/S1063074010010098

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1063074010010098

Key words

Search

Navigation