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Pathological Mitosis and Mixoploidy in the Meristematic Tissues of Grape Plant

  • DEVELOPMENTAL BIOLOGY OF PLANTS
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Abstract

The emergence of cells with pathological mitosis and the deviation from diploidity in the meristematic tissue of experimental plants of the genus Vitis (Tournef.) Linn. obtained from stenospermocarpic seeds was evaluated. The grape plants obtained from crossing seedless varieties with seedless and seed pollinators were the material for cytogenetic research. A total of 11 crosses were made. The cytogenetic analysis was carried out according to the recommended methods for fruit crops and grape. A total of 11 039 cells were examined. The pathology of mitosis in the cells of grape plants in vitro was observed, in particular, a hollow metaphase, a three-group metaphase and a metaphase with polar chromosomes as well as haploid, triploid, and tetraploid cells. The total frequency of abnormal cells was 0.15. The pathology of mitosis is observed in meristematic tissue of grape varieties as well. The evaluation of emergence of mitotic anomalies in the meristematic tissue of plants was carried out. The correspondence between the actual distribution of the anomalies in experimental populations and the theoretical distribution, as well as the degree of their relative habit to populations, the indices of absolute, relative, and intrapopulation diversity, was determined. As a result of the study of the polyembrionic hybrid offspring of grapes, differentiation in the equitability of distribution and habit of five mitotic anomalies to 11 populations was revealed, and differences between experimental populations by diversity were detected. The maximum degree of relative diversity of populations reached a value of 0.503. In general, the low relative diversity indicates a lack of complete equalization of anomalies by the presence in the populations.

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REFERENCES

  1. Alov, I.A., Tsitofiziologiya i patologiya mitoza (Cytophysiology and Pathology of Mitosis), Moscow: Meditsina, 1972.

  2. Jásek, J. and Hpudák, J., The electron microscopical study of the division of highly vacuolised grapevine callus cells, Biol. Plant., 1989, vol. 31, no. 5, pp. 321–326.

    Article  Google Scholar 

  3. Kalaev, V.N. and Butorina, A.K., Cytogenetic effect of radiation in seed of oak (Quercus robur L.) trees growing on sites contaminated by Chernobyl fallout, Silvae Genet., 2006, vol. 55, no. 3, pp. 93–101.

    Article  Google Scholar 

  4. Kalaev, V.N., Cytogenetic responses of deciduous woody plants to stress conditions and prospects of their use for assessment of environmental genotoxicity, Extended Abstract of Doctoral (Biol.) Dissertation, Voronezh: Voronezh. Gos. Univ., 2009.

  5. Kiselev, K.V., Lauve, L.S., and Tyunin, A.P., Chromosome variability in grape (Vitis amurensis Rupr.) cells transformed with plant oncogene rolB, Russ. J. Genet., 2013, vol. 49, no. 6, pp. 617–622.

    Article  CAS  Google Scholar 

  6. Klimenko, V.P., Metodicheskie rekomendatsii po kolichestvennoi genetike vinograda (Guidelines for Quantitative Genetics of Grapevine), Yalta: Inst. Vinograd. Vinodel. Magarach, 1998.

  7. Klimenko, V.P., Deviation from diploidy in cells of meristematic tissue of experimental grape plants derived from polyembryonic seeds, in Modern Biotechnology of Agricultural Plants and Biosafety: Abstracts of International Scientific Conference. Odessa, Ukraine, September 7–10, 2010, Odessa, 2010, p. 46.

  8. Klimenko, V.P., Scientific basics of creating the starting material and breeding new high-yield grape varieties, Extended Abstract of Doctoral (Agricult.) Dissertation, Yalta: Nauch. Inst. Vinograd. Vinodel. Magarach, 2014.

  9. Klimenko, V.P. and Pavlova, I.A., Distribution of rare morphs in populations of in vitro experimental grape plants derived from stenospermocarpic embryos, Fiziol. Biokhim. Kult. Rast., 2002, vol. 34, no. 5, pp. 406–412.

    Google Scholar 

  10. Klimenko, V.P., Pavlova, I.A., and Studennikova, N.L., The use of digital microscopy in grape breeding, Vinogradarstvo Vinodelie, 2008, vol. 38, pp. 12–14.

    Google Scholar 

  11. Klymenko, V. and Pavlova, I., Rare morphs into population in vitro plants developed from the wild grape embryos, in Plant Genetic Resources and Their Exploitation in the Plant Breeding for Food and Agriculture: Book of Abstracts 18th EUCARPIA Genetic Resources Section Meeting, Piešt’any, Slovak Republik, May 23–26, 2007, Piešt’any, 2007, p. 91–92.

  12. Kopytchuk, T.E. and Sechnyak, A.L., Regularity of mitosis in different varieties of winter bread wheat under the action of herbicides, Analele Universităţii din Oradea—Fascicula Biologie, 2012, vol. XIX, no. 1, pp. 80–83.

    Google Scholar 

  13. Korshikov, I.I. and Lapteva, O.V., Cytogenetic abnormalities of the Pinus pallasiana D. Don. (Pinaceae) seed germination on iron-ore dump in Kryvyi Rih region, Ukr. Bot. J., 2013, vol. 70, no. 5, pp. 683–688.

    Article  Google Scholar 

  14. Kozak, M.F. and Marchenko, N.V., Tsitogeneticheskie effekty vozdeistviya antropogennogo zagryazneniya vod nizhnei Volgi: monografiya (Cytogenetic Effects of Anthropogenic Pollution of Water of the Lower Volga: Monograph), Astrakhan: Izdatel’skii dom Astrakhanskii Universitet, 2008.

  15. Kuksova, V.B., Piven, N.M., and Gleba, Yu.Yu., Somaclonal variation and in vitro induced mutagenesis in grapevine, Plant Cell Tissue Organ Culture, 1997, vol. 49, no. 1, pp. 17–27.

    Article  CAS  Google Scholar 

  16. Kunakh, V.A., Genomic variability of somatic cells of plants, Biopolim. Kletka, 1995, vol. 11, no. 6, pp. 5–40.

    Google Scholar 

  17. Kurilo, L.F., Some mechanisms of induction of pathological mitoses with estradiol, Tsitologiya, 1969, no. 11 (12), pp. 1576–1580.

  18. Lakin, G.F., Biometriya (Bopmetry), Moscow: Vysshaya Shkola, 1990.

    Google Scholar 

  19. Marchenko, A.O., Golodriga, P.Ya., Klimenko, V.P., and Piven’, N.M., Somatic embryogenesis in grape tissue culture, Fiziol. Biokhim. Kult. Rast., 1987, vol. 19, no. 4, pp. 408–411.

    Google Scholar 

  20. Medvedeva, M.Yu. and Bolsunovskii, A.Ya., The range of chromosomal aberrations in root meristem of E. canadensis from the Yenisei River areas with different types of anthropogenic pollution, Ekol. Genet., 2016, vol. 14, no. 2, pp. 57–66.

    Google Scholar 

  21. Pavlova, I.A. and Klimenko, V.P., A method for obtaining grape plants from initial forms with low fertility, UA Patent no. 10662, 2005.

  22. Pierozzi, N.I., Karyotype and nor-banding of mitotic chromosomes of some Vitis L. species, Revista Brasileira de Fruticultura, Jaboticabal, 2011, vol. Especial, pp. 564–570.

  23. Pierozzi, N.I. and Moura, M.F., Karyotype analysis in grapevines, Revista Brasileira de Fruticultura, Jaboticabal—SP, 2016, vol. 38, no. 1, pp. 213–221.

  24. Rybin, V.A., Primenenie tsitologicheskogo metoda pri selektsionnoi rabote s plodovymi (The Use of Cytological Method in Breeding Work with Fruit Trees), Chisinau: Shtiintsa, 1962.

  25. Singh Ram, J., Plant Cytogenetics, Washington, DC: CRC Press LLC, 2003.

    Google Scholar 

  26. De Storme, N. and Mason, A., Plant speciation through chromosome instability and ploidy change: cellular mechanisms, molecular factors and evolutionary relevance, Curr. Plant Biol., 2014, no. 1, pp. 10–33.

  27. Timofeeva, O.A. and Rumyantseva, N.I., Kul’tura kletok i tkanei rastenii: uchebnoe posobie (The Culture of Cells and Tissues of Plants: Textbook), Kazan: Kazan. Fed. Univ., 2012.

  28. Topale, Sh.G., Poliploidiya u vinograda (Polyploidy in Grapevine), Chisinau: Shtiintsa, 1983.

  29. Topale, Sh., Kariologiya, poliploidiya i otdalennaya gibridizatsiya vinograda (Karyology, Polyploidy, and Distant Hybridization in Grapevine), Chisinau: Bot. Sad Akad. Nauk Moldovy, Nauch. Inst. Vinograd. Vinodel., 2011.

  30. Topale, Sh.G., Cytological study of local varieties and spontaneously arising tetraploid grapevine forms in Crimea, Magarach. Vinograd. Vinodel., 2015, no. 3, pp. 58–59.

  31. Tsolova, B. and Atanassov, A., Induction of polyembryony and secondary embryogenesis in culture for embryo rescue of stenospermocarpic genotypes of Vitis vinifera L., Vitis, 1994, vol. 33, pp. 55–56.

    Google Scholar 

  32. Viljoen, T.A. and Spies, J.J., Cytogenetical studies of three Vitis species, Vitis, 1995, vol. 34, no. 4, pp. 221–224.

    Google Scholar 

  33. Yang, X.M., Cao, Z.Y., An, L.Z., et al., In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L.), Euphytica, 2006, no. 152, p. 217. https://doi.org/10.1007/s10681-006-9203-7.

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ACKNOWLEDGMENTS

I thank I.A. Pavlova, PhD, for plant material, O.V. Adibekov for software, and O.G. Gavrish, PhD, for the inspiration of this work.

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  1. Magarach All-Russia National Research Institute for Viticulture and Winemaking, 298600, Yalta, Republic of Crimea, Russia

    V. P. Klimenko

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Correspondence to V. P. Klimenko.

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Translated by T. Borisova

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Klimenko, V.P. Pathological Mitosis and Mixoploidy in the Meristematic Tissues of Grape Plant. Russ J Dev Biol 50, 31–38 (2019). https://doi.org/10.1134/S1062360419020024

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