Abstract
The effect of low-dose radiation was studied on the growth dynamics of transfused Lewis carcinoma cells in female C57Bl/6 mice. Fourfold total fractionated irradiation was conducted at a dose of 75 mGy (at a dose rate of 0.154 Gy/min and a voltage of 200 kV), starting from day 10 after the transplantation with a four-day interval. On days 14 and 22, a group of mice was euthanized to study the expression levels of genes and noncoding RNAs (microRNAs and long noncoding RNAs) in tumor cells, as well as in normal tissues (bone marrow, liver, spleen, thymus). The total RNA was obtained, and complementary DNA was synthesized according to the manufacturer’s protocol. After that, a real-time PCR reaction was performed using the SYBR Green I dye (Thermo Scientific, United States) or a TaqMan probe and specific primers. Genes and noncoding RNAs were divided into groups of oncogenes and oncosuppressors according to scientific research. In the group of irradiated mice, a decrease in the tumor growth rate was observed, mainly from day 20, accompanied by a change in the activity of the studied genes. The ratio of the activity of oncosuppressors to oncogenes was calculated for the obtained tissues. These fractions were 0.5 for the tumor, 10.7 for the bone marrow, and 2.4 for the spleen and thymus. Thus, it can be stated that in the tumor there was a particularly pronounced activation of oncogenes compared to the activity of oncosuppressors, while the opposite effect was observed in normal tissues.
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This work was carried out on the basis of the Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency with the support of the JSC Science and Innovation program for the development of nuclear medicine of the Rosatom State Corporation and on the topic of the Vavilov Institute of General Genetics, Russian Academy of Sciences (State Assignment no. 0112-2019-0002).
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Mikhailov, V.F., Saleeva, D.V., Shulenina, L.V. et al. The Сonnection between the Growth Dynamics of Transplanted Lewis Carcinoma in Mice and Changes in the Activity of Genes and Noncoding RNAs after Low-Dose Irradiation. Biol Bull Russ Acad Sci 49, 2266–2278 (2022). https://doi.org/10.1134/S1062359022120135
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DOI: https://doi.org/10.1134/S1062359022120135