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Analysis of Actinobiota in the Tobacco Rhizosphere with a Heterologous Choline Oxidase Gene from Arthrobacter globiformis

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Abstract

Plants Nicotiana tabacum L. with the choline oxidase gene (codA) responsible for the synthesis of glycine-betaine by agrobacterial transformation were obtained. In order to identify possible environmental consequences during the cultivation of transformants, microbial complexes in the rhizosphere were studied. Soil actinomycetes were used as a bioindication group of microorganisms. The abundance, taxonomic composition, and functional structure of the rhizosphere complexes of the original Samsun variety and independent transgenic lines CodA7 and CodA17 with the different codA gene were expressed. There were no significant differences in the taxonomic structure or the occurrence of phytopathogenic fungal antagonists or cellulolytic agents in the rhizosphere of the original plants and transformants. The structure of actinomycete complexes of transformants was characterized by variability comparable in magnitude with variability due to the stress effect of the soil background.

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Funding

This work was carried out within the framework of State Assignments (project nos. 0767-2019-0090 and 0574-2019-0002) and was supported in part by the Russian Foundation for Basic Research, project no. 19-016-00207_a.

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Authors and Affiliations

  1. Rudnitskii Federal Agricultural Research Center of the Northeast, 610007, Kirov, Russia

    I. G. Shirokikh & Ya. I. Nasarova

  2. Vyatka State University, 610000, Kirov, Russia

    I. G. Shirokikh

  3. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    G. N. Raldugina

  4. All-Russia Research Institute of Agricultural Biotechnology, 127550, Moscow, Russia

    A. A. Gulevich & E. N. Baranova

Authors
  1. I. G. Shirokikh
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  2. Ya. I. Nasarova
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  3. G. N. Raldugina
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  4. A. A. Gulevich
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  5. E. N. Baranova
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Correspondence to I. G. Shirokikh.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Shirokikh, I.G., Nasarova, Y.I., Raldugina, G.N. et al. Analysis of Actinobiota in the Tobacco Rhizosphere with a Heterologous Choline Oxidase Gene from Arthrobacter globiformis. Biol Bull Russ Acad Sci 49, 713–720 (2022). https://doi.org/10.1134/S1062359022010137

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  • DOI: https://doi.org/10.1134/S1062359022010137

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