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Spectrophotometric determination of methimazole in pharmaceutical, serum and urine samples by reaction with potassium ferricyanide-Fe(III)

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Abstract

This paper describes a novel method to determine methimazole by spectrophotometry using a potassium ferricyanide-Fe(III) reaction. The study indicates that at pH 4.0 Fe(III) is reduced to Fe(II) by methimazole and in situ formed Fe(II) reacts with potassium ferricyanide to give soluble Prussian Blue which is characterized by means of XRD analysis. The absorbance of Prussian Blue is measured at the absorption maximum of 735 nm, and the amount of methimazole can be determined based on this absorbance. Beer’s law is obeyed in the range of methimazole concentrations of 0.02–6.00 μg/mL. The equation of the linear regression is A = −0.0058 + 0.49988c (μg/mL), with a correlation coefficient of 0.9998 and RSD of 0.80%. The detection limit (3σ/k) is 0.015 μg/mL, and the apparent molar absorption coefficient of indirect determination of methimazole is 5.7 ± 104 L/mol cm. This method has been successfully applied to the determination of methimazole in pharmaceutical, serum and urine samples, and average recoveries are in the range of 98.6–102.4%. Analytical results obtained with this novel method are satisfactory.

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Authors and Affiliations

  1. College of Chemistry and Environmental Science, Henan Normal University Xinxiang, Henan, 453007, P.R. China

    Chunhong Dong, Li Guo & Quan-min Li

  2. College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology Xinxiang, Henan, 453003, P.R. China

    Yan Zhang

  3. Department of Chemistry and Engineering, Jiaozuo University Jiaozuo, Henan, 454003, P.R. China

    Chunhong Dong

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  1. Chunhong Dong
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  2. Yan Zhang
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  3. Li Guo
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  4. Quan-min Li
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Dong, C., Zhang, Y., Guo, L. et al. Spectrophotometric determination of methimazole in pharmaceutical, serum and urine samples by reaction with potassium ferricyanide-Fe(III). J Anal Chem 65, 707–712 (2010). https://doi.org/10.1134/S1061934810070099

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  • DOI: https://doi.org/10.1134/S1061934810070099

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