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Discrimination of NPC cell lines associated with malignant types using Raman spectroscopy

  • Laser Spectroscopy
  • Published:
Laser Physics

Abstract

Nasopharyngeal carcinoma (NPC) is subdivided into four categories according to both the characterization of the NPC cell and their radiotherapy reaction. To discriminate these kinds of NPC, the representative cultured cell types (CNE1, CNE2, 5-8F, and 6-10B) are explored by single-cell micro-Raman spectroscopy (RS). The collected spectra are analyzed and compared by principal component analysis (PCA) and linear discriminate analysis (LDA). The algorithm is shown to have the lowest specificity and sensitivity of 89 and 92% for NPC division. These results demonstrate that it is a potentially clinical application for Raman spectroscopy to diagnosis NPC tissue types.

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References

  1. W. Fang, X. Li, Q. Jiang, Z. Liu, H. Yang, S. Wang, S. Xie, Q. Liu, T. Liu, and J. Huang, J. Transl. Med. 6, 32 (2008).

    Article  Google Scholar 

  2. E. T. Chang and H. O. Adami, Cancer Epidemiol., Biomarkers Prev. 15, 1765 (2006).

    Article  Google Scholar 

  3. Z. Q. Li, Y. F. Xia, Q. Liu, W. Yi, X. F. Liu, F. Han, W. Luo, and T. X. Lu, Int. J. Radiat. Oncol., Biol., Phys. 66, 1011 (2006)

    Article  Google Scholar 

  4. H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, Laser Phys. 21, 1122 (2011).

    Article  ADS  Google Scholar 

  5. Y. S. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, J. Raman Spectrosc. 35, 525 (2004).

    Article  ADS  Google Scholar 

  6. I. Vlasova and A. Saletsky, Laser Phys. 19, 2219 (2009).

    Article  ADS  Google Scholar 

  7. W. H. Sebastian, W. Tyler, and H. Thomas, Curr. Opin. Biotechnol. 20, 63 (2009).

    Article  Google Scholar 

  8. L. Su, Y. F. Sun, Y. Chen, P. Chen, A. G. Shen, X. H. Wang, J. Jia, Y. F. Zhao, X. D. Zhou, and J. M. Hu, Laser Phys. 22, 311 (2012).

    Article  Google Scholar 

  9. M. V. P. Chowdary, K. K. Kumar, J. Kurien, S. Mathew, and C. M. Krishna, Biopolymers. 83, 556 (2006).

    Article  Google Scholar 

  10. M. D. Keller, E. M. Kanter, and M. J. Anita, Spectroscopy. 21, 33 (2006).

    Google Scholar 

  11. V. Backman, Early Diagnosis of Cancer Using Light Scattering Spectroscopy (Massachusetts Institute of Technology, 2001).

  12. Y. Ye, Y. Chen, Y. Li, Y. Su, C. Zou, L. Ou, R. Chen, and H. Zeng, Spectrosc. Int. J. 25, 217 (2011).

    Article  Google Scholar 

  13. R. Chen, H. Huang, L. Q. Sun, J. J. Pan, W. W. Chen, Y. Su, S. Y. Feng, and Y. Z. Li, in Conference on Optics in Health Care and Biomedical Optics IV, Beijing, China, 18–20 October 2010, Proceedings of SPIE (SPIE-INT, Bellingham, 2010), vol. 7845.

    Google Scholar 

  14. J. Lin, R. Chen, S. Feng, Y. Li, Z. Huang, S. Xie, Y. Yu, M. Cheng, and H. Zeng, Biosens. Bioelectron. 25, 388 (2009).

    Article  Google Scholar 

  15. D. P. Lau, Z. W. Huang, H. Lui, C. S. Man, K. Berean, M. D. Morrison, and H. S. Zeng, Lasers Surg. Med. 32, 210 (2003).

    Article  Google Scholar 

  16. S. Feng, J. Lin, M. Cheng, Y. Z. Li, G. Chen, Z. Huang, Y. Yu, R. Chen, and H. Zeng, Appl. Spectrosc. 63, 1089 (2009).

    Article  ADS  Google Scholar 

  17. S. Y. Feng, R. Chen, J. Q. Lin, J. J. Pan, G. N. Chen, Y. Z. Li, M. Cheng, Z. F. Huang, J. Chen, and H. S. Zeng, Biosens. Bioelectron. 25, 2414 (2010).

    Article  Google Scholar 

  18. H. M. Wang, X. Y. Wu, Y. F. Xia, and J. Y. Qian, Chin. J. Cancer. 22, 579 (2003).

    Google Scholar 

  19. J. Li, Y. Fan, J. Chen, K. T. Yao, and Z.X. Huang, Cancer Genet. Cytogenet. 196, 23 (2010).

    Article  Google Scholar 

  20. Z. M. Zhang, S. Chen, Y. Z. Liang, Z. X. Liu, Q. M. Zhang, L. X. Ding, F. Ye, and H. Zhou, J. Raman Spectrosc. 41, 659 (2010).

    Article  ADS  Google Scholar 

  21. Z. M. Zhang, S. Chen, and Y. Z. Liang, Analyst (Cambridge, U.K.) 135, 1138 (2010).

    Article  ADS  Google Scholar 

  22. S. B. Kim, C. Temiyasathit, K. Bensalah, A. Tuncel, J. Cadeddu, W. Kabbani, A. V. Mathker, and H. Liu, Expert Syst. Appl. 37, 3863 (2010).

    Article  Google Scholar 

  23. A. Nijssen, T. C. B. Schut, F. Heule, P. J. Caspers, D. P. Hayes, M. H. Neumann, and G. J. Puppels, J. Invest. Dermatol. 119, 64 (2002).

    Article  Google Scholar 

  24. M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. W. Huang, Analyst (Cambridge, U.K.) 135, 3162 (2010).

    Article  ADS  Google Scholar 

  25. Z. W. Huang, S. K. Teh, W. Zheng, K. Lin, K. Y. Ho, M. Teh, and K. G. Yeoh, Biosens. Bioelectron. 26, 383 (2010).

    Article  Google Scholar 

  26. J. W. Chan, D. S. Taylor, T. Zwerdling, S. M. Lane, K. Ihara, and T. Huser, Biophys. J. 90, 648 (2006).

    Article  ADS  Google Scholar 

  27. K. W. Short, S. Carpenter, J. P. Freyer, and J. R. Mourant, Biophys. J. 88, 4274 (2005).

    Article  Google Scholar 

  28. M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Y. So, and Z. W. Huang, Int. J. Cancer. 128, 2673 (2011).

    Article  Google Scholar 

  29. R. G. Brereton, Chemometrics: Data Analysis for the Laboratory and Chemical Plant (Wiley, New York, 2003).

    Google Scholar 

  30. T. Ikoma, H. Kobayashi, J. Tanaka, D. Walsh, and S. Mann, Int. J. Biol. Macromol. 32, 199 (2003).

    Article  Google Scholar 

  31. S. K. Teh, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, and Z. W. Huang, J. Biomed. Opt. 13, 034013 (2008).

    Article  ADS  Google Scholar 

  32. S. K. Teh, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, and Z. Huang, Br. J. Surg. 97, 550 (2010).

    Article  Google Scholar 

  33. Z. W. Huang, M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, and K. G. Yeoh, J. Biomed. Opt. 15, 037017 (2010).

    Article  ADS  Google Scholar 

  34. J. H. Hughes, C. J. Leigh, S. S. Raab, S. Y. Hook, M. B. Cohen, and M. J. Suhrland, Cancer Cytopathol. 84, 289 (1998).

    Google Scholar 

  35. K. Maeda, Y. S. Chung, S. Takatsuka, Y. Ogowa, N. Onoda, T. Sawada, Y. Kato, A. Nitta, Y. Arimoto, and Y. Kondo, Br. J. Cancer. 72, 319 (1995).

    Article  Google Scholar 

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Authors and Affiliations

  1. MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou, 510631, Guangdong, China

    P. Liu, J. H. Li, Z. Y. Guo & Z. F. Zhuang

  2. Department of Radiation Oncology, Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou, 510060, China

    Z. W. Tu & Y. F. Xia

Authors
  1. P. Liu
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  2. J. H. Li
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  3. Z. W. Tu
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  4. Z. Y. Guo
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  5. Z. F. Zhuang
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  6. Y. F. Xia
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Corresponding author

Correspondence to Z. F. Zhuang.

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Original Text © Astro, Ltd., 2012.

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Liu, P., Li, J.H., Tu, Z.W. et al. Discrimination of NPC cell lines associated with malignant types using Raman spectroscopy. Laser Phys. 22, 1682–1688 (2012). https://doi.org/10.1134/S1054660X12110096

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  • DOI: https://doi.org/10.1134/S1054660X12110096

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