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Search for Candidate Genes for Mutations Disrupting Synaptonemal Complex Formation in the Sequenced Genome of Rye Secale cereale

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Abstract

The formation of a synaptonemal complex between homologous chromosomes during prophase I of meiosis is of great importance for the normal course of the recombination process. Disruptions in the formation of the synaptonemal complex can lead to both asynapsis (in this case, univalents will be present at the metaphase I stage) and heterologous synapsis (both univalents and multivalents will be detected at the metaphase I stage). Previously, we obtained rye mutants in which no formation of synaptonemal complexes (sy1 and sy9) was observed or the synapsis was heterologous (sy10, sy18, and sy19). We performed a bioinformatics analysis of the annotated rye genome and identified potential candidate genes for each of these mutants. The candidate genes were chosen based microsatellite mapping data and their comparison with annotated sequences of the rye genome. As a result, the following genes were selected: Mei2-like for the sy1 mutant, MAD2 for the sy9 mutant, BUB3.3 and BUB3.1 for sy10 and sy18, respectively, and Meiosis 5 for sy19.

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Funding

The study was carried out within the state task of the Institute of Genetics, Russian Academy of Sciences (topic no. 0092-2022-0002).

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, St. Petersburg Branch, 199034, St. Petersburg, Russia

    J. V. Sopova

  2. St. Petersburg State University, 199034, St. Petersburg, Russia

    J. V. Sopova, P. A. Zykin & S. P. Sosnikhina

  3. Institute of Genetics and Cytology, National Academy of Sciences of Belarus, 220072, Minsk, Republic of Belarus

    T. V. Dolmatovich

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  1. J. V. Sopova
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  2. P. A. Zykin
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  3. T. V. Dolmatovich
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  4. S. P. Sosnikhina
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Correspondence to J. V. Sopova.

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Sopova, J.V., Zykin, P.A., Dolmatovich, T.V. et al. Search for Candidate Genes for Mutations Disrupting Synaptonemal Complex Formation in the Sequenced Genome of Rye Secale cereale. Russ J Genet 59, 729–731 (2023). https://doi.org/10.1134/S1022795423070128

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