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Comparative Analysis of Transgenic Tobacco Plants with Different Heterologic Plant Defensive Genes

  • PLANT GENETICS
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Abstract

In order to study the possibility of creating new plant forms resistant to phytopathogens, a collection of transgenic plants of model tobacco culture with new different plant protective genes was obtained by the agrobacterial transformation method. First an addition of a collection with serine proteinase inhibitor BWI-1a (ISP) from buckwheat with fragments of a spidroin gene as putable enhancer by vector constructions different designs was done. Secondly, transgenic plants with an antimicrobial peptide from sinthetic wheat Triticum kiharae and with defensine from Stellaria media. Comparative study of physiological characteristics of transgenic plants in biotests in vivo (with isolated leaves) and in vitro (with well biotests) was carried out. Regardless of the design of the vector construction, the target genes were expressed to a different extent in the tissues of all transgenic plants and their seed and vegetative progenies and gave their tissues antibacterial activity, indicating the synthesis of the functional protein. The introduction to the tobacco tissues of the heterologic plant protective genes of different nature that plants use in different defense mechanisms led to a similar increase in antibacterial activity of the transgenic tobacco tissues.

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Funding

This work was carried out within the scope of governmental program no. 0112-2019-0002, “The Genetic Technologies in Biology, Medicine, and Agricultural and Nature Management.” The study was partially supported by the Russian Foundation for Basic Research (RFBR) and RFBR-Belarus, project nos. 19-016-00069 and 18-54-00008 respectively, as well as by the Russian Science Foundation, project no. 16-16-00032 (the designing of constructions to express the AMP genes of wheat T. kiharae).

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia

    N. V. Khadeeva, E. Yu. Yakovleva, T. V. Korostyleva, E. A. Istomina, T. I. Odintsova & A. M. Kudryavtsev

  2. Belozersky Institute of Physicochemical Biology, Moscow State University, 119992, Moscow, Russia

    Ya. E. Dunaevsky & M. A. Belozersky

  3. State Research Institute of Genetics and Selection of Industrial Microorganisms, 117545, Moscow, Russia

    K. V. Sydoruk & V. G. Bogush

Authors
  1. N. V. Khadeeva
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  2. E. Yu. Yakovleva
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  3. T. V. Korostyleva
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  4. E. A. Istomina
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  5. Ya. E. Dunaevsky
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  6. K. V. Sydoruk
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  7. M. A. Belozersky
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  8. T. I. Odintsova
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  9. V. G. Bogush
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  10. A. M. Kudryavtsev
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Corresponding author

Correspondence to T. V. Korostyleva.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Bibov

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Khadeeva, N.V., Yakovleva, E.Y., Korostyleva, T.V. et al. Comparative Analysis of Transgenic Tobacco Plants with Different Heterologic Plant Defensive Genes. Russ J Genet 56, 307–316 (2020). https://doi.org/10.1134/S1022795420030084

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  • DOI: https://doi.org/10.1134/S1022795420030084

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