Skip to main content
SpringerLink
Log in

Distribution of HLA DRB1, DQA1 and DQB1 Allelic Main Groups in the Vojvodina Province of Serbia: Genetic Relatedness with Other Populations

  • HUMAN GENETICS
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The Human Leukocyte Antigen (HLA) system represents a distinctive marker in identifying population groups since they exhibit a very high level of polymorphism that reveals remarkable frequency variation in human populations. In this study the gene frequencies of HLA class II DRB1, DQB1 and DQA1 alleles were studied in 100 randomly chosen individuals from region of Vojvodina, Serbia, with an aim to establish the genetic relationship between Vojvodina population and selected populations of European and non-European descent. DNA low resolution typing for DRB1, DQA1 and DQB1 was done using a standardized PCR-SSOP. Genetic distances between Vojvodina and 28 populations were computed based on frequency of DRB1 alleles. Phylogenetic trees were constructed using the neighbour-joining algorithm. The distribution of the observed genotypes is in Hardy–Weinberg equilibrium for all studied systems. The most frequent alleles were HLA-DQA1*01 (0.475), HLA-DQB1*05 (0.3), HLA-DRB1*11 (0.14). Two locus haplotype analysis identified HLA-DQB1*05-DQA1*01 (0.22), HLA-DQB1*03-DQA1*05 (0.135) and HLA-DQB1*06-DQA1*01 (0.1075) as the common haplotypes in Vojvodina population. Compared to other populations, Vojvodina appear to be genetically related to Balkan population, in particular Croatia, Slovenia and Bosnia and Herzegovinia. In conclusion, genetic distance matrix estimated using alleles at HLA-DRB1 locus, indicates that Vojvodina population is related to present-day nearby populations, Polish, Russians, Germans and French but not to Albanians from Kosovo and Bulgarians.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.

Similar content being viewed by others

REFERENCES

  1. Albalushi, K.R., Sellami, M.H., AlRiyami, H., Varghese, M., Boukef, M.K., and Hmida, S., HLA Class II (DRB1 and DQB1) polymorphism in Omanis, J. Transplant. Technol. Res., 2014, vol. 4. no. 2, pp. 134—139. https://doi.org/10.4172/2161-0991.1000134

    Article  Google Scholar 

  2. Poluektov, Y.O., Kim, A., and Sadegh-Nasseri, S., HLA-DO and its role in MHC class II antigen presentation, Front. Immunol., 2013, vol. 4, pp. 260—266. https://doi.org/10.3389/fimmu.2013.00260

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Tsai, S. and Santamaria, P., MHC class II polymorphisms, autoreactive T-cells, and autoimmunity, Front. Immunol., 2013, vol. 4, pp. 321—328. https://doi.org/10.3389/fimmu.2013.00321

  4. Robinson, J., Halliwell, J.A., Hayhurst, J.H., Flicek, P., Parham, P., and Marsh, S.G.E., The IPD and IPD-IMGT/HLA database: allele variant databases Nucleic Acids Res., 2015, vol. 43, pp. D423—D431.

    Article  CAS  PubMed  Google Scholar 

  5. Matei, L., Panaitescu, E., and Dogăroiu, C., A comparison of the HLA database of the Romanian population with the Balkan populations, Rom. J. Leg. Med., 2012, vol. 20, no. 3, pp. 223—228.

    Article  Google Scholar 

  6. Djulejić, E., Senev, A., Kirijas, M., Hristomanova, S., Petlichkovski, A., Trajkov, D., and Spiroski, M., Allele frequency of HLADQB1 locus in Macedonian population, Maced. J. Med. Sci., 2012, vol. 5, no. 1, pp. 67—71.

    Article  Google Scholar 

  7. Hajjej, A., Almawi, W.Y., Hattab, L., El-Gaaied, A., and Hmida, S. HLA class I and class II alleles and haplotypes confirm the Berber origin of the present day Tunisian population, PLoS One, 2015, vol.10, no. 8. e0136909. https://doi.org/10.1371/journal.pone.0136909

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Peterson, T., Bielawny, T., Lacap, P., Hardie, R., Daniuk, C., Mendoza, L., Thavaneswaran, S., Kariri, T., Kimani, J., Wachihi, C., Kimani, M., Ball, T., Plummer, F., and Luo, M., Diversity and frequencies of HLA class I and class II genes of an East African population. Open J. Genet., 2014, vol. 4, no. 2, pp. 99—124. https://doi.org/10.4236/ojgen.2014.42013

    Article  Google Scholar 

  9. Nikbin, B., Nicknam, M., Hadinedoushan. H., Ansaripour, B., Moradi, B., Yekaninejad, M., Aminikhah, M., Ranjbar, M., and Amirzargar, A., Human Leukocyte Antigen (HLA) class I and II polymorphism in Iranian healthy population from Yazd province, Iran. J. Allergy, Asthma Immunol., 2017, vol. 16, no. 1, pp. 1—13.

    Google Scholar 

  10. Kocsis, K. and Kocsis-Hodosi, E., Ethnic Geography of the Hungarian Minorities in the Carpathian Basin, Budapest: Simon, 2001.

    Google Scholar 

  11. Popović, J.D., Srbi u Vojvodini, Novi Sad: Matica srpska, 1990.

  12. Njegovan, D., Prisajedinjenje Vojvodine Srbiji, Novi Sad: Muzej Vojvodine Novi Sad, 2004.

    Google Scholar 

  13. Radonić, J., Srbija i Ugarska u srednjem veku, zbornik Vojvodina, Novi Sad: Prometej, 2008.

    Google Scholar 

  14. Stojšin, S., Ethnic diversity of population in Vojvodina at the beginning of the 21st century, Eur. Q. Polit. Attitudes Mentalities, 2015, vol. 4, no. 2, pp. 25—37.

    Google Scholar 

  15. Cady, N.C., Stelick, S, and Batt, C.A., Nucleic acid purification using microfabricated silicon structures, Biosens. Bioelectron., 2003, vol.19, no. 1, pp. 59—66.

    Article  CAS  PubMed  Google Scholar 

  16. Bugawan, T.L., Begovich, A.B., and Erlich, H.A., Rapid HLA-DPB typing using enzymatically amplified DNA and nonradioactive sequence-specific oligonucleotide probes, Immunogenetics, 1990, vol. 32, no. 4, pp. 231—241.

    Article  CAS  PubMed  Google Scholar 

  17. Meyer, D., Single, R., Mack, S.J., Lancaster, A., Nelson, M.P., Erlich, H.A., Fernandez-Vina, M., and Thomson, G., 13th IHWS Anthropology/Human Genetic Diversity Joint Report, chapter 4: Single locus polymorphism of classical HLA genes, Immunobiology of the Human MHC, Hansen, J.A., Ed., Seattle: International Histocompatibility Working Group Press, 2006, pp. 653—704.

  18. Gonzalez-Galarza, F.F., Takeshita, L.Y., Santos, E.J., Kempson, F., Maia, M.H., Silva, A.L., Ghattaoraya, G.S., Alfirevic, A., Jones, A.R., and Middleton, D., Nucleic Acids Res., 2015, vol. 28, pp. D784—D788. http://allelefrequencies.net/.

    Article  CAS  Google Scholar 

  19. Cavalli-Sforza, L.L. and Edwards, A.W.F., Phylogenetic analysis: models and estimation procedures, Am. J. Hum. Genet., 1967, vol. 19, no. 3, part 1, pp. 233—257.

  20. Kumar, S., Stecher, G., and Tamura, K., MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets, Mol. Boil. Evol., 2016, vol. 33, no. 7, pp. 1870—1874.

    Article  CAS  Google Scholar 

  21. Ćirković, S., Srbi među evropskim narodima, Beograd: Equilibrium, 2004.

    Google Scholar 

  22. Rathika, C., Murali, V., Dhivakar, M., Kamaraj, R., Padma Malini, R., Ramgopal, S., and Balakrishnan, K., Susceptible and protective associations of HLA alleles and haplotypes with cervical cancer in South India, Asian Pac. J. Cancer Prev., 2016, vol. 17, no. 5. pp. 2491—249.

    PubMed  Google Scholar 

  23. Kuranov, A.B., Vavilov, M.N., Abildinova, G.Z., Akilzhanova, A.R., Iskakova, A.N., Zholdybayeva, E.V., Boldyreva, M.N., Müller, C.A., and Momynaliev, K.T., Polymorphisms of HLA-DRB1, -DQA1 and -DQB1 in inhabitants of Astana, the capital city of Kazakhstan, PLoS One, 2014, vol. 9, no. 12. e115265. https://doi.org/10.1371/journal.pone.0115265

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Amirzargar, A., Mytilineos, J., Farjadian, S., Doroudchi, M., Scherer, S., and Opelz, G., Human leukocyte antigen class II allele frequencies and haplotype association in Iranian normal population, Hum. Immunol., 2001, vol. 62, no.11, pp. 1234—1238.

    Article  CAS  PubMed  Google Scholar 

  25. Jazairi, B., Khansaa, I., Ikhtiar, A., and Murad, H., Frequency of HLA-DRB1 and HLA-DQB1 alleles and haplotype association in Syrian population, Immunol. Invest., 2016, vol. 45, no. 2, pp. 172—179.

    Article  CAS  PubMed  Google Scholar 

  26. Stojanović, T., Balkanska civilizacija, Beograd: Centar za Geopoetiku, 1995.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, 21000, Novi Sad, Serbia

    S. I. Vojvodić & D. S. Ademović-Sazdanić

  2. University of Novi Sad, Medical faculty, 21000, Novi Sad, Serbia

    S. I. Vojvodić

Authors
  1. S. I. Vojvodić
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. S. Ademović-Sazdanić
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. I. Vojvodić.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vojvodić, S.I., Ademović-Sazdanić, D.S. Distribution of HLA DRB1, DQA1 and DQB1 Allelic Main Groups in the Vojvodina Province of Serbia: Genetic Relatedness with Other Populations. Russ J Genet 55, 124–130 (2019). https://doi.org/10.1134/S1022795419010150

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795419010150

Keywords:

Search

Navigation