Abstract
The influence of N-acetylcysteine (ACC) on the cytogenetic effects of etoposide in F1 CBA × C57BL/6 mice was studied. Etoposide introduced intraperitoneally in doses of 10, 20, 40, and 60 mg/kg has a dose-dependent clastogenic activity and has an aneugenic effect with the induction of mainly hypohaploid oocytes. ACC significantly decreases the aneugenic and clastogenic activity of etoposide (20 mg/kg) in oocytes of 6-, 9-, and 12-week-old mice during triple introduction at a dose 200 mg/kg per os. The most pronounced anticlastogenic ACC activity (an 80% decrease) was registered in 9-week-old females; a 100% decrease in aneugenesis was detected in 6-week-old female mice.
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References
Durnev, A.D. and Seredenin, S.B., Mutageny: skrining i farmakologicheskaya profilaktika vozdeistvii (Mutagens: Screening and Pharmacological Prevention of Effects), Moscow: Meditsina, 1998.
Yauk, C.L., Aardema, M.J., Benthem, J., et al., Approaches for identifying germ cell mutagens: report of the 2013 IWGT workshop on germ cell assays, Mutat. Res., 2015, vol. 783, pp. 36–54. doi 10.1016/jmrgentox.2015.01.008
Pligina, K.L., Zhanataev, A.K., Chaika, Z.V., and Durnev, A.D., Method of cytogenetic assay of mouse oocytes, Bull. Exp. Boil. Med., 2013, vol. 156, no. 1, pp. 114–118. doi 10.1007/s10517-013-2292-0
Morozova, T. and Andrushchishina, T., Acetylcysteine in clinical practice, Vrach, 2007, no. 12, pp. 37–38.
De Flora, S., Cesarone, C.F., Balansky, R.M., et al., Chemopreventive properties and mechanisms of N-acetylcysteine: the experimental background, J. Cell Biochem., 1995, no. 22, pp. 33–41. doi 10.1002/jcb.240590806
De Flora, S., Izzotti, A., Albini, A., et al., Antigenotoxic and cancer preventive mechanisms of N-acetylL-cysteine, Cancer Chemoprevention: Promising Cancer Chemoprevention Agents, Humana Press, 2004, vol. 1, pp. 37–67. doi 10.1007/978-1-59259-767-3_3
Travis, L.B., The epidemiology of second primary cancers, Cancer Epidemiol. Biomarkers Prev., 2006, vol. 15, no. 11, pp. 2020–2026. doi 10.1158/1055-9965. EPI06-0414
Attia, S.M., Dominant lethal mutations of topoisomerase II inhibitors etoposide and merbarone in male mice: a mechanistic study, Arch. Toxicol., 2012, vol. 86, no. 5, pp. 725–731. doi 10.1007/s00204-0110799-6
Mailhes, J.B., Marchetti, F., George, L.P., and Barnhill, D.R., Preferential pericentric lesions and aneuploidy induced in mouse oocytes by the topoisomerase II inhibitor etoposide, Teratog. Carcinog. Mutagen, 1994, vol. 14, pp. 39–51. doi 10.1002/tcm.1770140106
Xiao-Meng Li, Chao Yu, Zhong-Wei Wang, et al., DNA topoisomerase II is dispensable for oocyte meiotic resumption but is essential for meiotic chromosome condensation and separation in mice, Biol. Reprod., 2013, vol. 89(5), no. 118, pp. 1–11. doi 10.1095/biolreprod.113.110692
Bromberg, K.D., Burgin, A.B., and Osheroff, N., A two-drug model for etoposide action against human topoisomerase II, J. Biol. Chem., 2003, vol. 278, no. 9, pp. 7406–7412. doi 10.1074/jbc.M212056200
Gómez, R., Viera, A., Berenguer, I., et al., Cohesin removal precedes topoisomerase IIa-dependent decatenation at centromeres in male mammalian meiosis II, Chromosoma, 2014, vol. 123, nos. 1–2, pp. 129–146. doi 10.1007/s00412-013-0434-9
Sukhacheva, T.V., Bogush, T.A., and Kolomiets, O.L., Destructive effect of DNA topoisomerase II inhibitor vepesid on mouse spermatogenesis, Bull. Exp. Biol. Med., 2003, vol. 135, no. 5, pp. 464–469. doi 10.1023/A:1024919510096
Liu, J., Liu, M., Ye, X., et al., Delay in oocyte aging in mice by the antioxidant N-acetyl-L-cysteine (NAC), Hum. Reprod., 2012, vol. 27, no. 5, pp. 1411–1420. doi 10.1093/humrep/des019
Hsin, I.L., Sheu, G.T., Chen, H.H., et al., N-Acetyl cysteine mitigates curcumin-mediated telomerase inhibition through rescuing of Sp1 reduction in A549 cells, Mutat. Res., 2010, vol. 688, nos. 1–2, pp. 72–77. doi 10.1016/jmrfmmm.2010.03.011
Sato, N., Mizumoto, K., and Kusumoto, M., Up-regulation of telomerase activity in human pancreatic cancer cells after exposure to etoposide, Br. J. Cancer, 2000, vol. 82, no. 11, pp. 1819–1826. doi 10.1054/bjoc.2000.1117
Grdina, D.J., Murley, J.S., and Roberts, J.C., Effects of thiols on topoisomerase-II alpha activity and cell cycle progression, Cell Proliferation, 1998, vol. 31, nos. 5–6, pp. 217–229. doi 10.1111/j.1365-2184.1998tb01199x
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Original Russian Text © K.L. Pligina, A.K. Zhanataev, A.V. Kulakova, Z.V. Chaika, A.D. Durnev, 2016, published in Genetika, 2016, Vol. 52, No. 2, pp. 215–220.
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Pligina, K.L., Zhanataev, A.K., Kulakova, A.V. et al. Influence of acetylcysteine on cytogenetic effects of etoposide in mouse oocytes. Russ J Genet 52, 188–193 (2016). https://doi.org/10.1134/S1022795415110125
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DOI: https://doi.org/10.1134/S1022795415110125