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Effects of static magnetic field pretreatment with and without PEG 6000 or NaCl exposure on wheat biochemical parameters

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Abstract

In this study, the static magnetic field (SMF) flux of 2.9–4.7 mT was applied to mature embryo explants of Tekirdag and Selimiye wheat cultivars (Triticum aestivum L.) under tissue culture conditions at three different pretreatment times of 0, 2.2, and 19.8 s, at the rate of 1 m/s with and without 60 g/L PEG 6000 or 100 mM NaCl. Changes in different biochemical parameters were investigated. SMF pretreatment with and without 60 g/L PEG 6000 or 100 mM NaCl increased chlorophyll and carotenoid contents, FRAP values, and antioxidant enzyme (SOD, CAT, POX, and APX) activities in all experimental groups except for the activities of Mn-SOD in the cv. Selimiye root samples. Among SOD isozymes, Fe-SOD was affected by SMF stronger than other izozymes. The combinations of SMF pretreatment with and without 60 g/L PEG 6000 or 100 mM NaCl differently affected SOD isozyme activities besides its effects on other biochemical parameters.

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Abbreviations

APX:

ascorbate peroxidase

CAR:

carotenoids

CAT:

catalase

Chl:

chlorophyll; ferric reducing ability of plasma

MF:

magnetic field

NBT:

nitro blue tetrazolium

POX:

peroxidase

SMF:

static MF

SOD:

superoxide dismutase

TPTZ:

2,4,6-tri(2-pyridyl)-s-triazine

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Authors and Affiliations

  1. Department of Biology, Istanbul University, Faculty of Science, Vezneciler, Istanbul, 34143, Turkey

    A. Sen & S. Alikamanoglu

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  1. A. Sen
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  2. S. Alikamanoglu
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Correspondence to A. Sen.

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Sen, A., Alikamanoglu, S. Effects of static magnetic field pretreatment with and without PEG 6000 or NaCl exposure on wheat biochemical parameters. Russ J Plant Physiol 61, 646–655 (2014). https://doi.org/10.1134/S1021443714050148

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  • DOI: https://doi.org/10.1134/S1021443714050148

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