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Cadmium effects on mineral nutrition and stress in Potamogeton crispus

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Abstract

The mechanisms of plant tolerance to cadmium stress were studied by short-term exposure of Potamogeton crispus L. to various concentrations of Cd ranging from 0 to 0.09 mM. The accumulation of Cd and its influence on nutrient elements, chlorophyll pigments, ultrastructure, proline and MDA contents, and free radical production, as well as the activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR) were investigated. The higher Cd concentration in the medium resulted in a significant enhancement of Cd accumulation. Photosynthetic pigment content decreased and ultrastructural damage to the leaf cells was aggravated with the increase in the Cd concentrations. Disruption of chloroplasts and mitochondria was observed even at the lowest concentration of Cd. Meantime, the rate of O *−2 generation and the contents of H2O2 and MDA significantly increased under Cd stress, suggesting that Cd caused oxidative stress. In addition, the antioxidant system was clearly activated following Cd exposure. SOD and POD activities increased initially and then decreased, while APX and GR activities markedly increased. Simultaneously, mineral nutrition was disturbed. While K, P, Ca, and Cu contents decreased, Na, Fe, and Mn contents increased. Induction of antioxidant enzyme activities in leaves exposed to elevated Cd concentrations may be involved in Cd tolerance of P. crispus.

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Abbreviations

APX:

ascorbate peroxidase

Car:

carotenoids

Chl a :

chlorophyll a

Chl b :

chlorophyll b

GR:

glutathione reductase

PBS:

phosphate buffered saline

POD:

peroxidase

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Jiangsu Key Lab of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, 210046, P.R. China

    H. Y. Yang, G. X. Shi, Q. S. Xu & H. X. Wang

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  1. H. Y. Yang
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  2. G. X. Shi
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  3. Q. S. Xu
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  4. H. X. Wang
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Correspondence to G. X. Shi.

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This text was submitted by the authors in English.

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Yang, H.Y., Shi, G.X., Xu, Q.S. et al. Cadmium effects on mineral nutrition and stress in Potamogeton crispus . Russ J Plant Physiol 58, 253–260 (2011). https://doi.org/10.1134/S1021443711020245

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