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Behavior of potato gametoclonal plants against the necrotic strain of potato Y potyvirus

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Abstract

A total of 59 Solanum tuberosum androgenetic plants have been obtained through anther culture, 47 of which derived from a tetraploid clone, seven from a diploid hybrid, and five from an anther-derived clone. About two thirds of the anther-derived plants were dihaploids, a few were monohaploids (5.08%) or aneuploids (6.78%), whereas the tetraploid genotype generated about a third of tetraploids. Seven hundred twenty seven R1 plants arisen from tubers of the androgenetic potatoes were mechanically inoculated with the necrotic strain of the potato Y potyvirus (PVYN) and grown in a glasshouse. Fifty days after inoculation, the presence of PVYN in R1 plants was detected by DAS-ELISA (Double Autibody Sandwich). Only three plants (0.4%) of genotype H2-258 exhibited local necrotic symptoms (hypersensitivity reaction) suggesting the presence of the N y gene, and this extreme resistance is epistatic to hypersensitive resistance. The immunity (R y-gene) to PVYN was retained through anther culturing and present at all levels of ploidy. The pattern of segregation for immunity was differentiated according to the ploidy level of the anther-derived plants. This changed segregation pattern may be due to a loss of resistance during the culturing, when an endoreduplication has taken place or to the possible regeneration from Second-division restituted unreduced microspores. Anyway, this segregation pattern must be taken into account when gametoclones are used in genetic studies.

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Abbreviations

DAS:

double antibody sandwich

MS:

Murashighe and Skoog nutrient medium

PVYN :

necrotic strain of potato Y potyvirus

SDR:

second-division restitution

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Authors and Affiliations

  1. Department of Crop Science, School of Agricultural Technology, Technological Education Institute, P.O. Box 1939, Heraklion, GR 710 04, Crete, Greece

    G. Grammatikaki

  2. Plant Virus Laboratory, National Agricultural Research Foundation, Crete, Greece

    A. Avgelis

  3. National Agency for New Technologies, Energy and the Environment, Rome, Italy

    A. Sonnino

Authors
  1. G. Grammatikaki
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  2. A. Avgelis
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  3. A. Sonnino
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Additional information

Published in Russian in Fiziologiya Rastenii, 2007, Vol. 54, No. 4, pp. 572–578.

The text was submitted by the authors in English.

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Grammatikaki, G., Avgelis, A. & Sonnino, A. Behavior of potato gametoclonal plants against the necrotic strain of potato Y potyvirus. Russ J Plant Physiol 54, 507–512 (2007). https://doi.org/10.1134/S1021443707040115

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  • DOI: https://doi.org/10.1134/S1021443707040115

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