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Development of Bacterial Cellulose-Hyaluronic Acid Multicomponent Hydrogels via Click Chemistry for Biomedical Applications

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Abstract

The cross-linking of bacterial cellulose (BC), hydroxypropyl methylcellulose (HPMC), and hyaluronic acid (HA) accomplished with the Copper (I) catalyzed azide-alkyne cycloaddition (CuAAC) click reaction was investigated to obtain the multicomponent hydrogels that allow selective control of material properties. Click chemistry is one of many design methodologies that can be used to create hydrogels for multi-component systems. BC, HPMC, and HA are the most preferred and suitable natural polymers for tissue engineering studies. However, in order to combine their properties in a synergistic way, they must be functionalized to interact with each other. In this study, azide-functional HA was achieved by the reaction of hyaluronic acid with 1-azido-2,3-epoxypropane. To obtain multicomponent hydrogel alkyne-terminated cellulose is also prepared. The cellulose and hyaluronic acid functionalization was confirmed by using FTIR and NMR analysis. The crosslinking reaction took place at ambient conditions for 24 h. It was observed that the swelling capacity of the multi-component hydrogel declined with the increasing amount of BC due to its high crosslinking degree. In addition, the porous morphology of hydrogels makes them suitable for wound dressing applications. SEM results revealed that pore size and porosity decreased with increasing BC concentration. MTT assay demonstrated that the hydrogels promote cell proliferation and adhesion for 3T3 cells. In vitro cell culturing within the hydrogel demonstrated good cell spreading.

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REFERENCES

  1. H. Dong, L. Zheng, P. Yu, Q. Jiang, Y. Wu, C. Huang, and B. Yin, ACS Sustainable Chem. Eng. 8, 256 (2020).

    Article  CAS  Google Scholar 

  2. R. Shah, R. Vyroubal, H. Fei, N. Saha, T. Kitano, and P. Saha, AIP Conf. Proc. 1662, 040007 (2015).

  3. T. Carvalho, G. Guedes, F. L. Sousa, C. S. R. Freire, and H. A. Santos, Biotechnol. J. 14, 1900059 (2019).

  4. S. Tang, K. Chi, H. Xu, Q. Yong, J. Yang, and J. M. Catchmark, Carbohydr. Polym. 252, 117123 (2021).

  5. M. N. Collins and C. Birkinshaw, Carbohydr. Polym. 92, 1262 (2013).

    Article  CAS  PubMed  Google Scholar 

  6. L. Wang, S. Dong, Y. Liu, Y. Ma, J. Zhang, Z. Yang, W. Jiang, and Y. Yuan, Polymers 12, 1138 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  7. O. Jeon, S. J. Song, K. J. Lee, M. H. Park, S. H. Lee, S. K. Hahn, S. Kim, and B. S. Kim, Carbohydr. Polym. 70, 251 (2007).

    Article  CAS  Google Scholar 

  8. L. A. Solchaga, J. E. Dennis, V. M. Goldberg, and A. I. Caplan, J. Orthop. Res. 17, 205 (1999).

    Article  CAS  PubMed  Google Scholar 

  9. T. Kutlusoy, B. Oktay, N. K. Apohan, M. Süleymanoğlu, and S. E. Kuruca, Int. J. Biol. Macromol. 103, 366 (2017).

    Article  CAS  PubMed  Google Scholar 

  10. L. A. Pérez, R. Hernández, J. M. Alonso, R. Pérez-González, and V. Sáez-Martínez, Biomedicine 9, 1113 (2021).

    Google Scholar 

  11. S. S. Han, H. Y. Yoon, J. Y. Yhee, M. O. Cho, H. E. Shim, J. E. Jeong, and S. W. Kang, Polym. Chem. 9, 20 (2018).

    Article  CAS  Google Scholar 

  12. B. Oktay, ChemistrySelect 3, 11737 (2018).

    Article  CAS  Google Scholar 

  13. S. Akhan, B. Oktay, O. K. Özdemir, S. Madakbaş, and N. Kayaman-Apohan, Mater. Chem. Phys. 254, 123315 (2020).

  14. L. Zhou, Y. M. Zhai, M. B. Yang, and W. Yang, ACS Sustainable Chem. Eng. 7, 15617 (2019).

    Article  CAS  Google Scholar 

  15. V. G. Muir and J. A. Burdick, Chem. Rev. 121, 10908 (2021).

    Article  CAS  PubMed  Google Scholar 

  16. A. Uliniuc, M. Popa, T. Hamaide, and M. Dobromir, Cellul. Chem. Technol. 46, 1 (2012).

    CAS  Google Scholar 

  17. N. Pahimanolis, A. Sorvari, N. D. Luong, and J. Seppälä, Carbohydr. Polym. 102, 637 (2014).

    Article  CAS  PubMed  Google Scholar 

  18. O. Pinar, G. Bozdağ, İ. Yemişer, E. Büyük, and D. Kazan, Genet. Appl. 4, 38 (2020).

    Google Scholar 

  19. B. Łaskiewicz, J. Appl. Polym. Sci. 67, 1871 (1998).

    Article  Google Scholar 

  20. A. Lepetit, R. Drolet, B. Tolnai, D. Montplaisir, and R. Zerrouki, Polymer 126, 48 (2017).

    Article  CAS  Google Scholar 

  21. S. Manju and K. Sreenivasan, J. Colloid Interface Sci. 359, 318 (2011).

    Article  CAS  PubMed  Google Scholar 

  22. Y. Xi, T. Jiang, Y. Yu, J. Yu, M. Xue, N. Xu, and T. J. Webster, Int. J. Nanomed. 14, 6425 (2019).

    Article  CAS  Google Scholar 

  23. I. Youm, V. Agrahari, J. B. Murowchick, and B. B. C. Youan, Pharm. Res. 31, 2439 (2014).

    Article  CAS  PubMed  Google Scholar 

  24. B. Oktay, N. Kayaman-Apohan, M. Süleymanoğlu, and S. Erdem-Kuruca, Mater. Sci. Eng. C 73, 569 (2017).

    Article  CAS  Google Scholar 

  25. K. Sunitha, G. Unnikrishnan, and C. P. Reghunadhan Nair, Polym. Adv. Technol. 30, 435 (2019).

    Article  Google Scholar 

  26. K. Kabiri, H. Omidian, S. A. Hashemi, and M. J. Zohuriaan-Mehr, Eur. Polym. J. 39, 1341 (2003).

    Article  CAS  Google Scholar 

  27. M. N. Collins and C. Birkinshaw, J. Appl. Polym. Sci. 120, 1040 (2011).

    Article  CAS  Google Scholar 

  28. K. R. Coogan, P. T. Stone, N. D. Sempertegui, and S. S. Rao, Eur. Polym. J. 135, 109870 (2020).

  29. B. Oktay, E. Baştürk, N. Kayaman-Apohan, and M. V. Kahraman, Polym. Compos. 34, 1321 (2013).

    Article  CAS  Google Scholar 

  30. M. R. Singh, S. Patel, and D. Singh, Nanobiomater. Soft Tissue Eng. Appl. Nanobiomater. 5, 231 (2016).

    Article  CAS  Google Scholar 

  31. B. Oktay, N. Kayaman-Apohan, S. Erdem-Kuruca, and M. Süleymanoğlu, Polym. Adv. Technol. 26, 978 (2015).

    Article  CAS  Google Scholar 

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Funding

This work was supported by Marmara University, Commission of Scientific Research Project (M.U.BAPKO) under grant FEN-C-YLP-130319-0041.

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Authors and Affiliations

  1. Chemistry Department, Institute of Pure and Applied Sciences, Marmara University, 34720, Göztepe Kadiköy, Türkiye

    Şeyma Turan Okulmuş

  2. Chemistry Department, Faculty of Science, Marmara University, 34720, Göztepe Kadiköy, Türkiye

    Burcu Oktay & Nilhan Kayaman Apohan

  3. Bioengineering Department, Faculty of Engineering, Marmara University, 34720, Göztepe Kadiköy, Türkiye

    Dilek Kazan

Authors
  1. Şeyma Turan Okulmuş
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  2. Burcu Oktay
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  4. Nilhan Kayaman Apohan
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Correspondence to Dilek Kazan or Nilhan Kayaman Apohan.

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Şeyma Turan Okulmuş, Oktay, B., Kazan, D. et al. Development of Bacterial Cellulose-Hyaluronic Acid Multicomponent Hydrogels via Click Chemistry for Biomedical Applications. Polym. Sci. Ser. A 65, 682–691 (2023). https://doi.org/10.1134/S0965545X23600564

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  • DOI: https://doi.org/10.1134/S0965545X23600564

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