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Effect of the phase state of the lipid bilayer on the structure and characteristics of the polycation-(anionic liposome) complex

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Abstract

Interaction of the cationic polymer poly-N-ethyl-4-vinylpyridinium bromide with bilayer vesicles (liposomes) composed of zwitterionic dipalmitoylphosphatidylcholine and anionic cardiolipin (the molar fraction of the negatively charged cardiolipin groups is 0.2) is studied. The composition and characteristics of the polycation-liposome complex are shown to be controlled by the phase state of the lipid membrane. Liposomes whose membranes exist in their LC state (“liquid” liposomes) keep their integrity in the complex with polycation. The adsorbed polycation can be completely removed from the liposomal membrane by the addition excess amounts of a competing polyanion. The adsorption of polycation on the surface of liposomes whose membranes exist the gel state (“solid” liposomes) leads to the formation of defects in the membrane, and the polycation’s adsorption with such liposomes becomes irreversible. The defects that form are also preserved when solid liposomes on whose surface the polycation is sorbed are transformed into the liquid state. Moreover, the reversible contact between polycation and liquid liposomes becomes irreversible once the liposomal membranes bound to the polycation transform into the solid state.

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References

  1. Z. V. Leonenko, E. Finot, H. Ma, et al., Biophys. J. 86, 3783 (2004).

    Article  CAS  Google Scholar 

  2. A. Nikolova, R. Koynova, B. Tenchov, and D. Exerowa, Chem. Phys. Lipids 83, 111 (1996).

    Article  CAS  Google Scholar 

  3. I. G. Denisov, M. A. McLean, A. W. Shaw, et al., J. Phys. Chem., B 109, 15580 (2000).

    Article  Google Scholar 

  4. A. Filippov, G. Orädd, and G. Lindblom, Biophys. J. 86, 891 (2004).

    Article  CAS  Google Scholar 

  5. A. Hac, H. Seeger, M. Fidorra, and T. Heimburg, Biophys. J. 88, 317 (2005).

    Article  CAS  Google Scholar 

  6. R. Peters and R. J. Cherry, Proc. Natl. Acad. Sci. U. S. A. 79, 4317 (1982).

    Article  CAS  Google Scholar 

  7. G. Lenaz, Biosci. Rep. 7, 823 (1987).

    Article  CAS  Google Scholar 

  8. L. A. Bagatolli and E. Gratton, Biophys. J. 79, 434 (2000).

    Article  CAS  Google Scholar 

  9. Liposomes: A Practical Approach, Pract. Approach Ser., Ed. by B. D. Hames and E. Rickwood (Oxford Univ. Press, New York, 1990).

    Google Scholar 

  10. T. Berclaz and H. M. McConnel, Biochemistry 20, 6635 (1981).

    Article  CAS  Google Scholar 

  11. B. C. Rourke and A. G. Gibbs, J. Exp. Biol. 202, 3255 (1999).

    Google Scholar 

  12. J. Widomska, M. Raguz, and W. K. Subczynski, Biochim. Biophys. Acta, Biomembr. 1768, 2635 (2007).

    Article  CAS  Google Scholar 

  13. A. A. Yaroslavov, A. A. Efimova, V. I. Lobyshev, and V. A. Kabanov, Biochim. Biophys. Acta, Biomembr. 1560, 14 (2002).

    Article  CAS  Google Scholar 

  14. A. A. Yaroslavov, O. E. Kuchenkova, I. B. Okuneva, et al., Biochim. Biophys. Acta, Biomembr. 1611, 44 (2003).

    Article  CAS  Google Scholar 

  15. A. A. Yaroslavov and V. A. Kabanov, J. Controlled Release 78, 267 (2002).

    Article  Google Scholar 

  16. A. A. Yaroslavov, A. A. Efimova, V. I. Lobyshev, et al., Membr. Cell Biol. 10, 683 (1997).

    CAS  Google Scholar 

  17. A. A. Yaroslavov, E. A. Kiseliova, O. Yu. Udalykh, and V. A. Kabanov, Langmuir 14, 5160 (1998).

    Article  CAS  Google Scholar 

  18. R. M. Fuoss and U. P. Strauss, J. Polym. Sci. 3, 602 (1948).

    Article  CAS  Google Scholar 

  19. A. A. Yaroslavov, T. A. Sitnikova, A. A. Rakhnyanskaya, et al., Langmuir 23, 7539 (2007).

    Article  CAS  Google Scholar 

  20. A. A. Yaroslavov, A. A. Efimova, V. E. Kul’kov, and V. A. Kabanov, Polymer Science, Ser. A 36, 215 (1994) [Vysokomol. Soedin., Ser. A 36, 264 (1994)].

    Google Scholar 

  21. A. A. Yaroslavov, A. A. Efimova, V. I. Lobyshev, et al., Biol. Membr. 13, 628 (1996).

    CAS  Google Scholar 

  22. S. G. Starodubtzev, Yu. E. Kirsh, and V. A. Kabanov, Eur. Polym. J. 10, 739 (1997).

    Article  Google Scholar 

  23. A. A. Yaroslavov, V. Ye. Koulkov, E. G. Yaroslavova, et al., Langmuir 14, 5999 (1998).

    Article  CAS  Google Scholar 

  24. E. Tsuchida and K. Abe, Adv. Polym. Sci. 45, 1 (1982).

    Article  Google Scholar 

  25. V. A. Kabanov, Polymer Science, Ser. A 36, 143 (1994) [Vysokomol. Soedin., Ser. A 36, 183 (1994)].

    Google Scholar 

  26. H. Raghuraman and A. Chattopadhyay, Biophys. J. 87, 2419 (2004).

    Article  CAS  Google Scholar 

  27. M. Murata, S. Takahashi, S. Kagiwada, et al., Biochemistry 31, 1986 (1992).

    Article  CAS  Google Scholar 

  28. A. E. Gad, Biochim. Biophys. Acta, Biomembr. 728, 377 (1983).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Chemistry, Moscow State University, Moscow, 119991, Russia

    A. A. Yaroslavov, A. A. Efimova & A. V. Sybachin

Authors
  1. A. A. Yaroslavov
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  2. A. A. Efimova
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  3. A. V. Sybachin
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Correspondence to A. A. Yaroslavov.

Additional information

Original Russian Text © A.A. Yaroslavov, A.A. Efimova, A.V. Sybachin, 2009, published in Vysokomolekulyarnye Soedineniya, Ser. A, 2009, Vol. 51, No. 6, pp. 962–971.

This work was supported by the Russian Foundation for Basic Research, project no. 06-03-32907-a.

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Yaroslavov, A.A., Efimova, A.A. & Sybachin, A.V. Effect of the phase state of the lipid bilayer on the structure and characteristics of the polycation-(anionic liposome) complex. Polym. Sci. Ser. A 51, 638–647 (2009). https://doi.org/10.1134/S0965545X0906008X

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