Abstract
Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion (DI) experiment without countermeasures. To evaluate the humoral immunity function, we selected genes encoding Toll-like receptors; proteins mediating NF-kB, AP-1, Akt, and TLR4 pathways; apoptosis proteins; CLEC4E, HSPD1, and PTGS2 genes. Phenol-chloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLR function on the transcriptional level are observed from the earliest stages of the immune response to 21-d DI.
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This work was supported by the Russian Science Foundation, grant no. 18-75-10 086-P.
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The research program was approved at a meeting of the Academic Council (Minutes No. 6 dated June 27, 2018) and approved by the Commission on Biomedical Ethics at the State Scientific Center of the Russian Federation, IBMP RAS (Minutes No. 483 dated August 3, 2018). Volunteer testers passed the medical-expert commission and signed the informed consent to participate in the experiment in accordance with the Declaration of Helsinki.
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Translated by A. Deryabina
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Vlasova, D.D., Sadova, A.A., Galina, V.S. et al. Effect of 21-Day Dry Immersion on Expression of Inborn Immunity Genes Associated with the Toll-Like Receptors of Signaling Pathways. Hum Physiol 49, 807–814 (2023). https://doi.org/10.1134/S0362119723070174
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DOI: https://doi.org/10.1134/S0362119723070174