Abstract
The structural and geometric parameters of a silica matrix (SM) for the synthesis of heterosurface adsorbents (HAs) are optimized. Modification is performed by shielding the external surfaces of alkyl-modified silica (AS) using human serum albumin and its subsequent crosslinking. The structural and geometric characteristics of the SM, AS, and HA are measured via low-temperature nitrogen adsorption. It is found that the structural characteristics of AS pores with diameters D < 6 nm do not change during HA synthesis, while the volume of pores with diameters of 6 nm < D < 9 nm shrinks slightly due to the adsorption of albumin in the pore orifices. It is established that the volume of pores with diameters D > 9 nm reduces significantly due to adsorption of albumin. It is concluded that silica gel with a maximum pore size distribution close to 5 nm and a minimal proportion of pores with D > 9 nm is optimal for HA synthesis; this allows us to achieve the greatest similarity between the chromatographic retention parameters for HA and AS. The suitability of the synthesized adsorbents for analyzing drugs in biological fluids through direct sample injection is confirmed by chromatography. It was found that the percentage of the protein fraction detected at the outlet of the chromatographic column is 98%.
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References
J. Haginaka and J. Wakai, J. Chromatogr. A 596, 151 (1992).
O. González-Ortega, J. Porath, and R. Guzman, J. Chromatogr. A 1227, 115 (2012).
A. A. Serdan, S. M. Staroverov, S. Yu. Bogoslovskii, et al., USSR Inventor’s Certificate No. 1788463, Byull. Izobret., No. 2 (1993), p. 46.
A. A. Serdan, in 100 Years of Chromatography, Ed. by B. A. Rudenko (Nauka, Moscow, 2003), p. 570 [in Russian].
L. V. Saprykina, Cand. Sci. (Chem.) Dissertation (Moscow, 2008).
G. Félix and D. Campèse, Chromatografia 66, 759 (2007).
H. Uranishi, M. Nakamura, H. Nakamuro, Yu. Ikeda, et al., J. Chromatogr. B 879, 1029 (2011).
G. A. Fedorova and L. A. Kozhanova, in Chromatography for the Benefit of Russia, Ed. by A. A. Kurganov (Granitsa, Moscow, 2007), p. 666 [in Russian].
A. A. Serdan, S. Yu. Bogoslovskii, and P. N. Nesterenko, Russ. J. Phys. Chem. A 65, 1396 (1991).
S. J. Gregg and K. S. W. Sing, Adsorption, Surface Area, and Porosity (Academic Press, 1991).
Chemistry of Graft Surface Compounds, Ed. by G. V. Lisichkin (Fizmatlit, Moscow, 2003) [in Russian].
M. A. Kiselev, Yu. A. Gryzunov, G. E. Dobretsov, et al., Biophysics 46, 402 (2001).
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Original Russian Text © S.Yu. Bogoslovskii, A.A. Serdan, 2016, published in Zhurnal Fizicheskoi Khimii, 2016, Vol. 90, No. 3, pp. 451–455.
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Bogoslovskii, S.Y., Serdan, A.A. Optimizing heterosurface adsorbent synthesis for liquid chromatography. Russ. J. Phys. Chem. 90, 671–674 (2016). https://doi.org/10.1134/S0036024416030067
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DOI: https://doi.org/10.1134/S0036024416030067