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Morphological, Biochemical and Functional Changes in Sperm of the Siberian Sturgeon Acipenser baerii (Acipenseridae) during Its Short-Term Storage

  • STURGEONS (ACIPENSERIDAE)
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Abstract

The results of the study of factors affecting the duration of short-term storage of sperm of sturgeons (Acipenseridae) have been presented, using the example of the Siberian sturgeon Acipenser baerii, as well as morphological, biochemical and functional changes in sperm during such storage. Various short-term storage conditions were created, in which the storage temperature was 5–20°C, the spermatozoa concentration was 0.29–2.86 billion/mL, and the oxygen concentration was 2–12 mg O2/L. It has been established that by adjusting the temperature, the spermatozoa concentration, as well as the level of oxygen saturation of spermatozoa, it is possible to extend the viability of spermatozoa up to 10 days. However, the control of these parameters did not allow influencing the antioxidant activity, the growth of morphological abnormalities and heterotrophic protists in sperm. As a further direction in the development of methods for short-term storage of sturgeon sperm, it is recommended to search for safe preservatives that do not have toxic and mutagenic effects on spermatozoa.

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Notes

  1. Metodicheskiye ukazaniya po provedeniyu gematologicheskogo obsledovaniya ryb ot 02.02.99 g. No. 13-4-2-/1487 Ministerstva sel’skogo khozyaystva i prodovol’stviya RF (Guidelines for Conducting Hematological Examination of Fish of February 2, 1999, No. 13-4-2-/1487 of the Ministry of Agriculture and Food of the Russian Federation), Moscow: Departament Veterinarii, 1999.

REFERENCES

  1. Alavi, S.M.H., Hatef, A., Pšenička, M., et al., Sperm biology and control of reproduction in sturgeon: (II) sperm morphology, acrosome reaction, motility and cryopreservation, Rev. Fish Biol. Fish., 2012, vol. 22, no. 4, pp. 861–886. https://doi.org/10.1007/s11160-012-9270-x

    Article  Google Scholar 

  2. Aramli, M.S., Kalbassi, M.R., Nazari, R.M., et al., Effects of short-term storage on the motility, oxidative stress, and ATP content of Persian sturgeon (Acipenser persicus) sperm, Anim. Reprod. Sci., 2013, vol. 143, nos. 1–4, pp. 112–117. https://doi.org/10.1016/j.anireprosci.2013.10.010

    Article  CAS  Google Scholar 

  3. Baiandina, I.S. and Khanaychenko, A.N., Optimization of the method for determining the motility characteristics of fish spermatozoa using ImageJ software and excel macros, J. Ichthyol., 2019, vol. 59, no. 1, pp. 127–130. https://doi.org/10.1134/S0032945219010016

    Article  Google Scholar 

  4. Barulin, N.V., Serum enzyme response of captive sturgeon brookstock Acipenser baerii Brandt 1869 females and two hybrids (bester = female Huso huso Linnaeus, 1758 × male Acipenser ruthenus Linnaeus, 1758, and RsSs = A. gueldenstaedtii Brandt 1833 × A. baerii Brandt 1869) to hormonal stimulation for spawning induction, J. Appl. Ichthyol., 2015, vol, 31. no. s2, pp. 2–6. https://doi.org/10.1111/jai.12898

    Article  CAS  Google Scholar 

  5. Barulin, N.V., Intravital sex identification of adult sterlets Acipenser ruthenus (Acipenseridae) based on the morphological structure of dorsal scutes, J. Ichthyol., 2018, vol. 58, no. 1, pp. 17–30. https://doi.org/10.1134/S0032945218010022

    Article  Google Scholar 

  6. Barulin, N.V., Using machine learning algorithms to analyse the scute structure and sex identification of sterlet Acipenser ruthenus (Acipenseridae), Aquac. Res., 2019, vol. 50, no. 10, pp. 2810–2825. https://doi.org/10.1111/are.14233

    Article  Google Scholar 

  7. Barulin, N.V. and Shumski, K.L, Regulirovanie kachestva spermoproduktsii osetrovykh ryb v tekhnologii vosproizvodstva ob”ektov akvakul’tury (Regulation of the Quality of Sturgeon Sperm Production in the Technology of Reproduction of Aquaculture Objects), Gorki: Beloruss. Gos. Sel’.-Khoz. Akad., 2019.

  8. Borg, K.E., Lunstra, D.D., and Christenson, R.K., Semen characteristics, testicular size, and reproductive hormone concentrations in mature Duroc, Meishan, Fengjing and Minzhu boars, Biol. Reprod., 1993, vol. 49, no. 3, pp. 515–521. https://doi.org/10.1095/biolreprod49.3.515

    Article  CAS  Google Scholar 

  9. Cejko, B.I., Żarski, D., Sarosiek, B., et al., Application of artificial seminal plasma to short-term storage of a large volume of common carp (Cyprinus carpio) sperm for two weeks under controlled conditions, Aquaculture, 2022, vol. 546, Article 737385. https://doi.org/10.1016/j.aquaculture.2021.737385

    Article  Google Scholar 

  10. Chebanov, M.S. and Galich, E.V., Sturgeon Hatchery Manual, in FAO Fisheries and Aquaculture Techn. Pap., Ankara: Food Agric. Org., 2011, no. 558.

  11. Cheng, Y., Zhang, S., Linhartová, Z., et al., Practical use of extender and activation solutions for short-term storage of common carp (Cyprinus carpio) milt in a hatchery, Aquac. Rep., 2022, vol. 24, Article 101160. https://doi.org/10.1016/j.aqrep.2022.101160

    Article  Google Scholar 

  12. Collier, J.J., Chiotti, J.A., Boase, J., et al., Assessing habitat for lake sturgeon (Acipenser fulvescens) reintroduction to the Maumee River, Ohio using habitat suitability index models, J. Gt. Lakes Res., 2022, vol. 48, no. 1, pp. 219–228. https://doi.org/10.1016/j.jglr.2021.11.006

    Article  CAS  Google Scholar 

  13. Dass, S.A.H., Vasudevan, A., Dutta, D., et al., Protozoan parasite Toxoplasma gondii manipulates mate choice in rats by enhancing attractiveness of males, PLoS One, 2011, vol. 6, no. 11, Article e27229. https://doi.org/10.1371/journal.pone.0027229

    Article  CAS  Google Scholar 

  14. Dorsey, K.M., Guthrie, H.D., Welch, G.R., Mohler, J., et al., Quality assessment of wild Atlantic sturgeon semen under conditions of short-term storage, N. Am. J. Aquac., 2011, vol. 73, no. 4, pp. 418–425. https://doi.org/10.1080/15222055.2011.629945

    Article  Google Scholar 

  15. Fox, J., The R Commander: A basic statistics graphical user interface to R, J. Stat. Softw., 2005, vol. 14, no. 9, pp. 1–42. https://doi.org/10.18637/jss.v014.i09

    Article  Google Scholar 

  16. Gilroy, C.E. and Litvak, M.K., Relationship between seminal plasma composition and spermatozoa swimming speed and motility in wild and captive shortnose sturgeon (Acipenser brevirostrum), Aquaculture, 2019, vol. 505, pp. 217–224. https://doi.org/10.1016/j.aquaculture.2019.02.033

    Article  Google Scholar 

  17. Kruger, T.F., Ackerman, S.B., Simmons, K.F., et al., A quick, reliable staining technique for human sperm morphology, Arch. Androl., 1987, vol. 18, no. 3, pp. 275–277. https://doi.org/10.3109/01485018708988493

    Article  CAS  Google Scholar 

  18. Lahnsteiner, F. and Mansour, N., The effect of temperature on sperm motility and enzymatic activity in brown trout Salmo trutta, burbot Lota lota and grayling Thymallus thymallus, J. Fish. Biol., 2012, vol. 81, no. 1, pp. 197–209. https://doi.org/10.1111/j.1095-8649.2012.03323.x

    Article  CAS  Google Scholar 

  19. Le, M.H., Lim, H.K., Min, B.H., et al., Effects of varying dilutions, pH, temperature and cations on spermatozoa motility in fish Larimichthys polyactis, J. Environ. Biol., 2011, vol. 32, no. 3, pp. 271–276.

    Google Scholar 

  20. Liu, S., Su, Y., Yi, H., et al., Effect of short-term storage on sperm functional parameters in sex-reversed female mandarin fish (Siniperca chuatsi), Aquaculture, 2022, vol. 547, Article 737410. https://doi.org/10.1016/j.aquaculture.2021.737410

    Article  CAS  Google Scholar 

  21. Lopes, Franca C., Castro, J.D.J.P., Silva, J.M., et al., Characterization of short-term storage of the semen of Trachelyopterus galeatus (Siluriformes: Auchenipteridae) incorporating dimethyl sulphoxide (dmso) to the diluent, Aquac. Res., 2022, vol. 53, no. 6, pp. 2218–2226. https://doi.org/10.1111/are.15740

    Article  CAS  Google Scholar 

  22. Nascimento, J.P., Horokhovatskyi, Y., Kholodnyy, V., et al., Optimization of sterlet sperm concentration for cryopreservation, Aquaculture, 2021, vol. 540, Article 736682. https://doi.org/10.1016/j.aquaculture.2021.736682

    Article  Google Scholar 

  23. Pavlov, D.A., A method for the assessment of sperm quality in fish, J. Ichthyol., 2006, vol. 46, no. 5, Article 391. https://doi.org/10.1134/S0032945206050055

    Article  Google Scholar 

  24. Pérez-Crespo, M., Pintado, B., and Gutiérrez-Adán, A., Scrotal heat stress effects on sperm viability, sperm DNA integrity, and the offspring sex ratio in mice, Mol. Reprod. Dev., 2008, vol. 75, no. 1, pp. 40–47. https://doi.org/10.1002/mrd.20759

    Article  CAS  Google Scholar 

  25. Plavskii, V., Mikulich, A., Barulin, N., et al., Comparative effect of low-intensity laser radiation in green and red spectral regions on functional characteristics of sturgeon sperm, Photochem. Photobiol., 2020, vol. 96, no. 6, pp. 1294–1313. https://doi.org/10.1111/php.13315

    Article  CAS  Google Scholar 

  26. Plavskii, V.Y., Barulin, N.V., Mikulich, A.V., et al., Effect of continuous wave, quasi-continuous wave and pulsed laser radiation on functional characteristics of fish spermatozoa, J. Photochem. Photobiol. B. Biol., 2021, vol. 216, Article 112112. https://doi.org/10.1016/j.jphotobiol.2020.112112

    Article  CAS  Google Scholar 

  27. Pohlert, T., The Pairwise Multiple Comparison of Mean Ranks Package (PMCMR). R package, 2014. http://CRAN.R-project.org/package=PMCMR. Version 06/2022.

  28. Porporato, M., Grillone, G., Patetta, A., et al., Survey of the health status of some honey bee queens in Italy, J. Apic. Sci., 2015, vol. 59, no. 2, pp. 27–36. https://doi.org/10.1515/jas-2015-0022

    Article  Google Scholar 

  29. Sanocka, D. and Kurpisz, M., Reactive oxygen species and sperm cells, Reprod. Biol. Endocrinol., 2004, vol. 2, Article 12. https://doi.org/10.1186/1477-7827-2-12

    Article  Google Scholar 

  30. Shaliutina-Kolešová, A. and Nian, R., Motility and oxidative stress of common carp Cyprinus carpio sperm during short-term storage, Anim. Reprod. Sci., 2022, vol. 241, Article 106991. https://doi.org/10.1016/j.anireprosci.2022.106991

    Article  Google Scholar 

  31. Siddique, M.A.M., Psenicka, M., Cosson, J., et al., Egg stickiness in artificial reproduction of sturgeon: An overview, Rev. Aquac., 2016, vol. 8, no. 1, pp. 18–29. https://doi.org/10.1111/raq.12070

    Article  Google Scholar 

  32. Venables, W.N. and Ripley, B.D., Modern Applied Statistics with S, N.Y.: Springer, 2002. https://doi.org/10.1007/978-0-387-21706-2

  33. Wei, T. and Simko, V., Corrplot: Visualization of a Correlation Matrix. R package version 0.77, 2016. https://CRAN.R-project.org/package=corrplot. Version 06/2022.

  34. WHO Laboratory Manual for the Examination and Processing of Human Semen, Genewa: WHO, 2010.

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Funding

This work was supported by The Belarusian Republican Foundation for Fundamental Research (project B22U-011).

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  1. Belarusian State Agricultural Academy (BSAA, Gorki, Mogilev Region, Republic of Belarus

    N. V. Barulin & K. L. Shumski

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Translated by S. Avodkova

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Barulin, N.V., Shumski, K.L. Morphological, Biochemical and Functional Changes in Sperm of the Siberian Sturgeon Acipenser baerii (Acipenseridae) during Its Short-Term Storage. J. Ichthyol. 62, 1476–1489 (2022). https://doi.org/10.1134/S0032945222060030

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