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Electric and Viscoelastic Parameters of Erythrocytes in Models for Diagnostics of Adenomatous Polyps and Stages of Colorectal Cancer in Optical Detection of Cells in an Inhomogeneous Alternating Electric Field

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Abstract

Using the method of dielectrophoresis an optical cell detection system in an inhomogeneous alternating electric field, the electrical and viscoelastic parameters of red blood cells in the frequency range from 5 × 104 to 1 × 106 MHz have been studied in 129 patients with colorectal cancer (CRC) of various stages of the disease, 25 people with adenomatous polyps, and 35 people in the comparison group. It has been shown that the values of the physical characteristics of red blood cells significantly differ both when comparing the control groups and the patients with adenomatous polyps and the patients with early stages of CRC and when comparing the patients with different stages of the oncological process with each other. The construction of SHAP graphs based on the electrical and viscoelastic parameters of red blood cells has allowed us to achieve 100% diagnostic accuracy in distinguishing patients with adenomatous polyps and early stages of CRC from healthy individuals, 98.7% accuracy—in differentiating the patients with polyps from the patients with early stages of CRC, and 91.9% accuracy—in distinguishing early and late stages of CRC. The results obtained demonstrate the high potential of the proposed method for screening and diagnosing CRC.

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Funding

The work was carried out within the framework of the topic “Epidemiological monitoring of the state of public health and the study of molecular-genetic and molecular-biological mechanisms of the development of common therapeutic diseases in Siberia to improve approaches to their diagnosis, prevention, and treatment,” state assignment 0324-2018-0001, reg. no. AAAA-A17-117112850280-2.

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Authors and Affiliations

  1. Institute of Internal and Preventive Medicine, “The Federal Research Center Institute of Cytology and Genetics,” Siberian Branch, Russian Academy of Sciences, 630089, Novosibirsk, Russia

    M. V. Kruchinina, A. A. Gromov & L. V. Shcherbakova

  2. State Research Center of Virology and Biotechnology “Vector,” Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk oblast, Russia

    V. M. Generalov & K. V. Generalov

  3. Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    V. N. Kruchinin & S. V. Rykhlitskii

  4. Novosibirsk State Medical University, 630091, Novosibirsk, Russia

    M. V. Kruchinina & E. V. Kruchinina

  5. Novosibirsk State Technical University, 630073, Novosibirsk, Russia

    I. N. Yakovina & M. V. Yakovlev

  6. Siberian State University of Geosystems and Technologies, 630108, Novosibirsk, Russia

    O. V. Minin & I. V. Minin

Authors
  1. M. V. Kruchinina
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  2. A. A. Gromov
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  3. L. V. Shcherbakova
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  4. E. V. Kruchinina
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  5. V. M. Generalov
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  6. K. V. Generalov
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  7. V. N. Kruchinin
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  8. S. V. Rykhlitskii
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  9. I. N. Yakovina
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  10. M. V. Yakovlev
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  11. O. V. Minin
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  12. I. V. Minin
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Corresponding author

Correspondence to M. V. Kruchinina.

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All procedures performed in a human study comply with the ethical standards of the institutional and/or National committee on Research Ethics and the 1964 Helsinki Declaration and its subsequent amendments or comparable standards of ethics.

Conflict of interest. The authors state that they have no conflicts of interest.

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Translated by N. Petrov

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Kruchinina, M.V., Gromov, A.A., Shcherbakova, L.V. et al. Electric and Viscoelastic Parameters of Erythrocytes in Models for Diagnostics of Adenomatous Polyps and Stages of Colorectal Cancer in Optical Detection of Cells in an Inhomogeneous Alternating Electric Field. Opt. Spectrosc. 129, 1327–1340 (2021). https://doi.org/10.1134/S0030400X21060060

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