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Genes Associated with Increased Stress Sensitivity in Hypertensive ISIAH Rats

  • EVOLUTIONARY, POPULATION, AND MEDICAL GENOMICS, TRANSCRIPTOMICS
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Abstract

Inherited stress-induced arterial hypertension (ISIAH) rats are characterized by increased stress reactivity of the hypothalamic–pituitary–adrenal and sympathoadrenal systems. The genetic basis of increased susceptibility to stress was studied in hypertensive ISIAH rats. Adrenal transcriptomes were sequenced in hypertensive ISIAH and normotensive WAG rats, and nine differentially expressed genes (DEGs) were found in the X-chromosome locus that was previously associated with mild emotional stress-induced increases in blood pressure and plasma corticosterone and an increased adrenal weight in ISIAH rats. An analysis of the functions performed by DEG-encoded proteins suggested the Sms (spermine synthase) gene to be the most likely candidate gene in the X-chromosome locus associated with an elevated stress susceptibility in ISIAH rats.

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ACKNOWLEDGMENTS

We are grateful to JSC Genoanalytica (Russia) for help in transcriptome sequencing and primary bioinformatics analysis.

Funding

This work was supported by the Russian Science Foundation (project no. 22-14-00082).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    L. A. Fedoseeva, S. E. Smolenskaya, A. L. Markel & O. E. Redina

  2. Novosibirsk State University, 630090, Novosibirsk, Russia

    A. L. Markel

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  1. L. A. Fedoseeva
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  2. S. E. Smolenskaya
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  3. A. L. Markel
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  4. O. E. Redina
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L.A. Fedoseeva and S.E. Smolenskaya contributed equally to this study.

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Correspondence to O. E. Redina.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by T. Tkacheva

Abbreviations: ISIAH, inherited stress-induced arterial hypertension; WAG, Wistar Albino Glaxo; BP, blood pressure; QTL, quantitative trait locus; DEG, differentially expressed gene; PCR, polymerase chain reaction; RGD, Rat Genome Database.

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Fedoseeva, L.A., Smolenskaya, S.E., Markel, A.L. et al. Genes Associated with Increased Stress Sensitivity in Hypertensive ISIAH Rats. Mol Biol 57, 346–355 (2023). https://doi.org/10.1134/S0026893323020073

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  • DOI: https://doi.org/10.1134/S0026893323020073

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