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Kinetics of interactions between apomyoglobin and phospholipid membrane

  • Structural-Functional Analysis of Biopolymers and their Complexes
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Abstract

The interaction of apomyoglobin and its mutant forms with phospholipid membranes was studied using tryptophan fluorescence and circular dichroism in the far UV region. It is shown that a negatively charged phospholipid membrane can have a dual effect on the structure of protein molecule upon their interaction. On the one hand, the membrane induces denaturation of the protein native structure to its intermediate state, acting as a moderate denaturing agent. On the other hand, it can stabilize the structure of unfolded protein to the same intermediate state, acting as a moderate structuring agent. The kinetics of interaction between apomyoglobin and its mutant forms and the phospholipid membrane depends on the membrane surface charge. Here the interaction rate depends on the concentration of phospholipids vesicles and stability of protein molecule, which increase with a decrease in the latter. The roles of these factors in the folding of membrane proteins and the choice of the targeted delivery pathways for protein drugs are discussed.

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Abbreviations

WT apoMb:

wild-type apomyoglobin

PLs:

phospholipids

Cm :

the urea concentration in the middle of transition

POPG:

palmitoyl-oleoyl-phosphatidylglycerol

POPC:

palmitoyl-oleoyl-phosphatidylcholine

CD:

circular dichroism

UV:

ultraviolet

N:

native state

I:

intermediate state

U:

unfolded state

Trp:

tryptophan.

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Authors and Affiliations

  1. Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia

    V. A. Balobanov, N. B. Il’ina, N. S. Katina, I. A. Kashparov & V. E. Bychkova

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    D. A. Dolgikh

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  1. V. A. Balobanov
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  2. N. B. Il’ina
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  3. N. S. Katina
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  4. I. A. Kashparov
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  5. D. A. Dolgikh
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  6. V. E. Bychkova
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Correspondence to V. A. Balobanov.

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Original Russian Text © V.A. Balobanov, N.B. Il’ina, N.S. Katina, I.A. Kashparov, D.A. Dolgikh, V.E. Bychkova, 2010, published in Molekulyarnaya Biologiya, 2010, Vol. 44, No. 4, pp. 708–717.

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Balobanov, V.A., Il’ina, N.B., Katina, N.S. et al. Kinetics of interactions between apomyoglobin and phospholipid membrane. Mol Biol 44, 624–632 (2010). https://doi.org/10.1134/S0026893310040187

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