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Microbial Communities Associated with the White Sea Red Algae as a Source of Xylanolytic Microorganisms

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Abstract

Microorganisms associated with algae and able to utilize complex substrates (e.g., plant heteropolysaccharides) may be important producers of hydrolytic enzymes. The microbial communities of the red algae Corallina sp. and Phyllophora sp. sampled in the Kandalaksha Gulf basin of the White Sea were analyzed using high-throughput sequencing of the V4-variable region of the 16S rRNA gene. The dominant phyla in microbiomes of both samples were Pseudomonadota and Bacteroidota (GTDB classification, https://gtdb.ecogenomic.org/). For the Corallina sp. sample, dominance of the Vibrio, Agarivorans, and Photobacterium genera was shown, while Granulosicoccus and Aliivibrio dominated in the Phyllophora sp. sample. The analyzed red macroalgae with associated microbiota were used as an inocula to obtain microbial enrichment cultures growing on β-1,4-xylan or β-1,3-glucan (curdlan). It was shown that, similar to environmental samples Pseudomonadota and Bacteroidota phyla representatives were prevalent in all enrichment cultures. However, unlike the environmental samples, in the enrichment cultures the dominant genera were Marinomonas, Reinekea, Polaribacter, and Pseudoalteromonas. The latter, as well as the representatives of Vibrio sp., were isolated in pure cultures for which the xylanolytic activity was shown.

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ACKNOWLEDGMENTS

The authors are grateful to E.N. Vinogradova (Moscow State University) for assistance in identification of algae and in preparation of pure culture samples for sequencing, to A.Yu. Merkel (Research Center of Biotechnology) for assistance in statistical analysis of the data, and to A.B. Tzetlin, director of the Pertsov Biological Station, as well as to all the personnel of the station, for the provided possibility to use the research facilities of the station.

Funding

The study was supported by the Ministry of Science and Higher Education of the Russian Federation (agreement no. 075-15-2021-1396).

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Authors and Affiliations

  1. Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    V. D. Salova, A. M. Kholdina, A. D. Melnik & I. V. Kublanov

  2. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    K. S. Zayulina, A. G. Elcheninov, A. A. Klyukina & I. V. Kublanov

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  1. V. D. Salova
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Salova, V.D., Kholdina, A.M., Melnik, A.D. et al. Microbial Communities Associated with the White Sea Red Algae as a Source of Xylanolytic Microorganisms. Microbiology 92, 418–426 (2023). https://doi.org/10.1134/S002626172360026X

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