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Search for Efficient Chitosan-Based Fungicides to Protect the 15th‒16th Centuries Tempera Painting in Exhibits from the State Tretyakov Gallery

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Abstract—

Growth inhibition by chitosans (with molecular weight 6, 12, 18, 25 and 45 kDa) was demonstrated for 10 filamentous fungi belonging to the families Aspergillaceae, Cladosporiacea, Pleosporaceae, Cordycipitaceae and Microascaceae which are responsible for biodeterioration of tempera paintings and are the dominant members of microbiome from Paintings of Ancient Rus Halls, State Tretyakov Gallery, Russia. The greatest effect was achieved at 2% chitosan in agar medium; its antifungal activity increased in the row: 6‒12‒18‒25 kDa. Chitosans with a molecular weights of 25 and 45 kDa inhibited fungal growth to the same degree. Representatives of Cladosporiaceae and Sordariomycetes (Cordycipitaceae and Microascaceae) showed the greatest sensitivity to chitosan. Some Aspergillaceae were found to exhibit elevated resistance. Investigation of antifungal activity of chitosans incorporated into tempera materials to assess their possible application as art objects antiseptics will be the goal of our further research.

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Funding

The work was partially supported by the Russian Foundation for Basic Research, projects nos. 20-016-00205 (obtaining the chitosan samples) and 17-29-04349 (determination of antifungal activity). G.K. Nuraeva is grateful to the Ministry of Science and Higher Education of the Russian Federation for support of the work on cultivation of filamentous fungi within the framework of the state contract no. 075-00337-20-03 (project identifier FSMG-2020-0007).

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Authors and Affiliations

  1. Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    A. A. Zhgun, D. A. Avdanina, B. Ts. Shagdarova, M. P. Potapov, A. V. Il’ina & V. P. Varlamov

  2. State Tretyakov Gallery, 119017, Moscow, Russia

    E. V. Troyan & M. V. Shitov

  3. Moscow Institute of Physics and Technology (National Research University), 141700, Dolgoprudny, Moscow region, Russia

    G. K. Nuraeva

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  1. A. A. Zhgun
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  2. D. A. Avdanina
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  3. B. Ts. Shagdarova
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  4. E. V. Troyan
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  5. G. K. Nuraeva
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  6. M. P. Potapov
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  7. A. V. Il’ina
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  8. M. V. Shitov
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  9. V. P. Varlamov
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Correspondence to A. A. Zhgun.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Zhgun, A.A., Avdanina, D.A., Shagdarova, B.T. et al. Search for Efficient Chitosan-Based Fungicides to Protect the 15th‒16th Centuries Tempera Painting in Exhibits from the State Tretyakov Gallery. Microbiology 89, 750–755 (2020). https://doi.org/10.1134/S0026261720060193

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