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Changes in the phase variant spectra in the populations of lactic acid bacteria under antibiotic treatment

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Abstract

Effect of the antibiotics kanamycin and ampicillin on the growth and phase variation of the populations of four strains of lactic acid bacteria (Lactobacillus sp. M76AT, L. casei MB, Enterococcus faecium M, and E. faecium M3185) was studied. The presence of antibiotics in the medium resulted in a dose-dependent decrease in viable cell numbers and in partial or complete substitution of the dominant S variant with the minor Sm and Sb variants. The variants differed in colony morphology, as well as in some physiological, biochemical, biotechnological, and probiotic characteristics. The Sm type variants of all strains exhibited the highest resistance to antibiotics. High production of exopolysaccharides was found in Sb variants of lactobacilli and in S variants of enterococci. The highest antibacterial activity was found in Sm variants of lactobacilli, especially in Lactobacillus sp. M76AT. The latter is biotechnologically the most promising strain, since all variants fermented milk yielding the products with uniformly pronounced functional and organoleptic properties. These patterns are of importance for the understanding of the mechanisms of antibiotic resistance and for selection of the variants with desired properties, as well as for quality control of the lactic acid bacteria starter cultures.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    N. G. Loiko, A. N. Kozlova, Yu. A. Nikolaev, V. F. Gal’chenko & G. I. El’-Registan

  2. Moscow State University of Food Production, Moscow, Russia

    M. A. Krasnova, T. V. Pichugina, A. I. Grinevich & V. I. Ganina

Authors
  1. N. G. Loiko
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  2. M. A. Krasnova
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  3. T. V. Pichugina
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  4. A. I. Grinevich
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  5. V. I. Ganina
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  6. A. N. Kozlova
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  7. Yu. A. Nikolaev
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  8. V. F. Gal’chenko
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  9. G. I. El’-Registan
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Correspondence to N. G. Loiko.

Additional information

Original Russian Text © N.G. Loiko, M.A. Krasnova, T.V. Pichugina, A.I. Grinevich, V.I. Ganina, A.N. Kozlova, Yu.A. Nikolaev, V.F. Gal’chenko, G.I. El’-Registan, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 3, pp. 284–294.

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Loiko, N.G., Krasnova, M.A., Pichugina, T.V. et al. Changes in the phase variant spectra in the populations of lactic acid bacteria under antibiotic treatment. Microbiology 83, 195–204 (2014). https://doi.org/10.1134/S0026261714030114

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  • DOI: https://doi.org/10.1134/S0026261714030114

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