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Chemical composition and immunochemical characteristics of the lipopolysaccharide of nitrogen-fixing rhizobacterium Azospirillum brasilense CD

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Abstract

The chemical composition of the lipopolysaccharide of the associative diazotrophic rhizobacterium Azospirillum brasilense Cd has been studied. Among the main components of the hydrophobic part of the lipopolysaccharide, we identified 3-hydroxytetradecanoic, hexadecenoic, 3-hydroxyhexadecanoic, hexadecanoic, octadecenoic, and nanodecanoic fatty acids; the carbohydrate part contained rhamnose, galactose, and mannose. Polyclonal antibodies against the preparation under study were raised in rabbits. Serological relations between A. brasilense Cd and other strains of Azospirillum spp. were studied using double radial immunodiffusion and enzyme-linked immunosorbent assay.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia

    O. N. Konnova, G. L. Burygin, Yu. P. Fedonenko, L. Yu. Matora, S. A. Konnova & V. V. Ignatov

  2. Saratov State University, Saratov, Russia

    K. E. Pankin

Authors
  1. O. N. Konnova
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  2. G. L. Burygin
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  3. Yu. P. Fedonenko
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  4. L. Yu. Matora
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  5. K. E. Pankin
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  6. S. A. Konnova
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  7. V. V. Ignatov
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Correspondence to S. A. Konnova.

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Original Russian Text © O.N. Konnova, G.L. Burygin, Yu.P. Fedonenko, L.Yu. Matora, K. E. Pankin, S.A. Konnova, V.V. Ignatov, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 3, pp. 383–388.

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Konnova, O.N., Burygin, G.L., Fedonenko, Y.P. et al. Chemical composition and immunochemical characteristics of the lipopolysaccharide of nitrogen-fixing rhizobacterium Azospirillum brasilense CD. Microbiology 75, 323–328 (2006). https://doi.org/10.1134/S0026261706030143

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  • DOI: https://doi.org/10.1134/S0026261706030143

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