Abstract
Excessive long-term accumulation of calcium ions in the myoplasm of skeletal muscles can negatively affect mitochondria and lead to muscle dysfunction. The aim of our study was to identify the role of L-type calcium channels in the development of increased fatigue of the rat soleus muscle under functional unloading. Young male Wistar rats were divided into three groups of 8 animals each: vivarium control group (C), group exposed to 7-day hindlimb unloading (7HS), and group exposed to 7-day hindlimb unloading with daily intraperitoneal injections of nifedipine (7 mg/kg body weight) (7HS+N). Nifedipine administration during hindlimb unloading prevented the upregulation of calcium-dependent phosphorylation of calcium-calmodulin kinase II (CaMKII) and an increase in fatigue, as well as promoted the preservation of mitochondrial proteins, mtDNA, and mRNA expression of a number of regulatory genes of mitochondrial biogenesis in the rat soleus muscle.
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This work was supported by the Russian Science Foundation (RSF project no. 21-75-00063).
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Conceptualization and experimental design (B.S.Sh., K.A.Sh.), data collection (I.D.L., D.A.S., S.A.T.), data processing (I.D.L., K.A.Sh.), writing and editing the manuscript (B.S.Sh., K.A.Sh., I.D.L., D.A.S., S.A.T.)
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All applicable international, national, and/or institutional principles for the care and use of animals were followed. All experimental procedures performed with the involvement of animals complied with ethical standards approved by legal acts of the Russian Federation, the principles of the Basel Declaration, and the recommendations of the Biomedical Ethics Committee at the Institute of Biomedical Problems of the Russian Academy of Sciences (Minutes no. 629, 06.12.2022).
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Translated by A. Polyanovsky
Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 4, pp. 517–529https://doi.org/10.31857/S086981392304009X.
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Sharlo, K.A., Lvova, I.D., Tyganov, S.A. et al. Role of L-Type Calcium Channels in Increased Fatigue of the Rat Soleus Muscle under Functional Unloading. J Evol Biochem Phys 59, 620–629 (2023). https://doi.org/10.1134/S0022093023020278
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DOI: https://doi.org/10.1134/S0022093023020278