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A Raman Spectroscopic Study of the Conformation of Flavin Adenine Dinucleotide, a CoEnzyme of D-Amino Acid Oxidase

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Abstract

The spectra of surface-enhanced Raman scattering of D-amino acid oxidase from pig kidney were recorded and analyzed using silver nanoparticles; characteristic spectral parameters of changes in the conformation of the flavinadenine dinucleotide cofactor during activation of the enzyme by D-amino acids were revealed. It was found that the time during which changes in the conformation of the flavinadenine dinucleotide were recorded depended on the substrate specificity of the enzyme: in the presence of D-alanine, this took several seconds, and in the presence of D-serine, minutes.

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Funding

The research was carried out with the financial support of the Russian Science Foundation, project no. 23-74-00006 for Zh. V. Bochkova and N. A. Brazhe, as well as the Interdisciplinary Scientific and Educational School of Moscow State University Molecular technologies of living systems and synthetic biology.

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Authors and Affiliations

  1. Department of Biology, Moscow State University, 119234, Moscow, Russia

    J. V. Bochkova, N. A. Brazhe & G. V. Maksimov

  2. Department of Biology, MSU-BIT University, Dayun New Town, Longgang District, 518172, Shenzhen, Guangdong Province, China

    W. Liu

  3. Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071, Moscow, Russia

    A. A. Zhgun

Authors
  1. J. V. Bochkova
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  2. W. Liu
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  3. N. A. Brazhe
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  4. A. A. Zhgun
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  5. G. V. Maksimov
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Corresponding author

Correspondence to G. V. Maksimov.

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The authors of this work declare that they have no conflicts of interest.

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Translated by E. Puchkov

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Abbreviations: DAAO, D-amino acid oxidase; FAD, flavin adenine dinucleotide; RS, Raman spectroscopy; SERS, surface-enhanced Raman scattering.

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Bochkova, J.V., Liu, W., Brazhe, N.A. et al. A Raman Spectroscopic Study of the Conformation of Flavin Adenine Dinucleotide, a CoEnzyme of D-Amino Acid Oxidase. BIOPHYSICS 68, 719–724 (2023). https://doi.org/10.1134/S0006350923050068

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  • DOI: https://doi.org/10.1134/S0006350923050068

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