Abstract
The structural-functional characteristics of the cells of wild type CC-124 and Brc-1 mutant of the unicellular green algae Chlamydomonas reinhardtii grown in the dark and in the light were studied. The cells of the wild type in heterotrophic and mixotrophic conditions had a well developed structure and high functional activity due to the ability of the cells to synthesize chlorophyll both in the light and in the dark. The cells of Brc-1 mutant lost their ability to synthesize chlorophyll in the dark and the cell color was orange due to brc-1 mutation in the nuclear gene LTS3 that regulated the activity of Mg-chelatase enzyme. In the dark the mutant cells accumulated protoporphyrin IX and had weakly developed structure with low functional activity. Because of the high content of protoporphyrin IX, even a short-term exposure of the Brc-1 mutant cells to the light was accompanied by very strong destructive changes in all the membranes in a cell: plasmalemma, chloroplast, mitochondrion, envelopes of the nucleus and vacuoles. The causes of significant impairment of the membrane components and O2-gas exchange in the Brc-1 mutant cells are discussed.
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N. P. Yurina, O. V. Osipenkova, and M. S. Odintsova, Fiziol. Rast. 59, 3 (2012).
E. H. Harris, Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 52, 363 (2001).
G. A. Armstrong, J. Photochem. Photobiol. B: Biology 43, 87 (1998).
E. M. Chekunova, Ekol. Genetika, no. 3, 38 (2010).
E. M. Chekunova and V. G. Ladygin, Photosynthesis: Open Questions and What We Know Today, Ed. by S. I. Allakhverdiev, A. B. Rubin, V. A. Shuvalov (Inst. Komp. Issl., Moscow-Izhevsk, 2013), ch. 16, pp. 535–615 [in Russian].
C. Ford, S. Mitchell, and W. Y. Wang, Mol. Gen. Genet. 184, 460 (1981).
Sh. Zhang Identification of Novel “Yellow-In-The-Dark” Mutants in Chlamydomonas reinhardtii (University of Virginia, 2007).
E. M. Chekunova, E. B. Yaronskaya, N. V. Yartseva, and N. G. Averina, Fiziol. Rast. 61(2), 1 (2014).
W.-Y. Wang, W. L. Wang, J. E. Boynton, and N. E. Gillham, J. Cell Biology 63, 806 (1974).
E. M. Chekunova and K. V. Kvitko, Issled. Genetike, no. 10, 104 (1986).
N. V. Shalygo, E. M. Chekunova, A. S. Chunaev, and N. G. Averina, Izv. AN BSSR Ser. Biol., no. 4, 53 (1990).
E. Chekounova, V. Voronetskaya, J. Papenbrock, et al., Mol. Gen. Genet. 266, 363 (2001).
E. M. Chekunova and N. V. Savel’eva, Ekol. Genetika, no. 2, 35 (2010).
H. K. Lichtenthaller and A. R. Wellburn, Biochem. Soc. Trans. 11, 591 (1983).
H. K. Lichtenthaller, Methods Enzymol. 148, 331 (1987).
N. G. Averina, V. V. Sidorko, and N. V. Shalygo, Vestn. AN BSSR, no. 4, 102 (1984).
V. P. Egorov, G. M. Anan’ev, and A. A. Kosobryukhov, Experimental Ecology, Ed. by V. I. Kefeli, V. N. Kudeyarov (Nauka, Moscow, 1991), pp. 9–18 [in Russian].
J. L. Priol and P. Chartier, Annals of Botany 41, 789 (1977).
D. A. Walker, Philosophical Transaction of the Royal Society London. B 323, 313 (1989).
V. G. Ladygin and G. A. Semenova, Biophysics 58, 791 (2013).
V. G. Ladygin, Fiziol. Rast. 45(5), 741 (1998).
V. G. Ladygin, Mikrobiologiya 83(2), 160 (2014).
G. A. Semenova, E. P. Severina, and V. G. Ladygin, Fiziol. Rast. 34(1), 74 (1987).
S. V. Manuil’skaya, V. G. Ladygin, and A. I. Mikhno, Biokhimiya 52(5), 731 (1987).
V. M. Trusova, V. G. Ladygin, V. V. Mexentsev, and M. I. Molchanov, Dokl. AN SSSR 293(3), 751 (1987).
G. L. Klyachko-Gurvich, N. A. Pronina, V. G. Ladygin, et al., Fiziol. Rast. 38(6), 1171 (1991).
M. Seras, J. Garnier, A. Tremolieres, and D. Guyon, Plant Physiol. Biochem. 27(3), 393 (1989).
A. A. Krasnovskii, Progress in Porphyrin Chemistry, Ed. by O. A. Golubchikov (Izd. NII Khimiya SPbGU, St. Petersburg, 2001), vol. 3, pp. 191–216 [in Russian].
R. G. Op den Camp, D. Przybyla, C. Ochsenbein, et al., Plant Cell. 15(10), 2320 (2003).
N. V. Shalygo, Biosynthesis of Chlorophyll and Photodynamic Processes in Plants (Pravo i Ekonomika, Minsk, 2004)[in Russian].
M. N. Merzlyak and V. A. Kovrizhnykh, Studia Biophys. 102(1), 81 (1984).
J. N. Chacon, J. McLearie, and R. S. Sinclair, Photochem. Photobiol. 47(5), 647 (1988).
N. V. Shalygo, H.-P. Mock, N. G. Averina, and B. Grimm, J. Photochem. Photobiol. B: Biology. 42(2), 151 (1998).
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Original Russian Text © V.G. Ladygin, E.M. Chekunova, G.A. Semenova, A.A. Kosobryukhov, 2014, published in Biofizika, 2014, Vol. 59, No. 4, pp. 692–703.
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Ladygin, V.G., Chekunova, E.M., Semenova, G.A. et al. Structural-functional organization of the cells of Brc-1 mutant of Chlamydomonas reinhardtii accumulating protoporphyrin IX in the dark. BIOPHYSICS 59, 568–576 (2014). https://doi.org/10.1134/S0006350914040162
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DOI: https://doi.org/10.1134/S0006350914040162