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Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244

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Abstract

The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, suggesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane-bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone-synthesizing Pseudomonas putida HK5.

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Abbreviations

DAS:

dense alignment surface method

mGDH:

membrane-bound glucose dehydrogenase

PQQ:

pyrrolo-quinoline quinone

QDH:

quinate dehydrogenase

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    A. S. Vangnai & W. Promden

  2. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    W. De-Eknamkul

  3. Laboratory of Applied Microbiology, Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan

    K. Matsushita

  4. Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru, Nishihara-cho, Okinawa, 903-0213, Japan

    H. Toyama

Authors
  1. A. S. Vangnai
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  2. W. Promden
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  3. W. De-Eknamkul
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  4. K. Matsushita
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  5. H. Toyama
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Correspondence to A. S. Vangnai.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 4, pp. 549–557.

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Vangnai, A.S., Promden, W., De-Eknamkul, W. et al. Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244. Biochemistry Moscow 75, 452–459 (2010). https://doi.org/10.1134/S0006297910040085

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  • DOI: https://doi.org/10.1134/S0006297910040085

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