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Production of Lycopene by Fusarium chlamydosporum and its Anti-inflammatory Activity on Raw Macrophage Cell Line

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Abstract

The aim of the present study was to characterize the pigment from the fungus Fusarium chlamydosporum following the evaluation of its anti-inflammatory activity on RAW 264.7 macrophage cell line. The red pigment extracted from F. chlamydosporum was subjected to series of purification techniques wherein the pigment was identified to be lycopene. The cytotoxicity of the pigment as well as evaluation of anti-inflammatory activity was carried out on normal human lung fibroblast MRC-5 cell line and LPS-stimulated RAW 264.7 macrophage cell line, respectively. The inhibition of cytokines IL-1β and IL-6 was assessed by indirect ELISA. The gene expression of COX-2 and IL-1β in lycopene-treated cells was carried out by real-time PCR wherein the expressions of both the pro-inflammatory markers were found to be down-regulated. The study concludes the production of lycopene from F. chlamydosporum when grown in a complex medium. The pigment showed profound anti-inflammatory activity in vitro. The present findings of lycopene production by the fungus F. chlamydosporum can be a major break-through in the research field for the production of natural pigments with high yield against various ailments.

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ACKNOWLEDGMENTS

Authors acknowledge the Department of Microbiology, Bangalore University wherein I have carried out the current work.

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Authors and Affiliations

  1. Department of Microbiology, Krupanidhi Degree College, Bengaluru, India

    T. A. Thomas

  2. Department of Microbiology, Bangalore University, Bengaluru, India

    S. Tirumale

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  1. T. A. Thomas
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  2. S. Tirumale
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Correspondence to S. Tirumale.

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The authors declare that they have no conflicts of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

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Thomas, T.A., Tirumale, S. Production of Lycopene by Fusarium chlamydosporum and its Anti-inflammatory Activity on Raw Macrophage Cell Line. Appl Biochem Microbiol 59, 308–315 (2023). https://doi.org/10.1134/S0003683823030183

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