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Screening and Taxonomic Characterization of Xylanase Bacterial Producers

  • PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING
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Abstract

A stepwise screening of bacterial xylanase producers isolated from forest soil in Moscow oblast has been performed, and the taxa of the microorganisms have been identified based on an analysis of the 16S RNA gene sequences. An approach to the selection of the most active producers is suggested, and this selection has been carried out, resulting in four producing strains. Interestingly, the species of two of the selected strains (Paenibacillus jamilae and P. brasilensis) are described as producers of xylanolytic enzymes for the first time, and the characteristics of those xylanases have not yet been studied. The new strains can serve as a source of genes for the construction of high-efficient xylanase producers.

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Abbreviations

LB medium:

— Luria-Bertani medium

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ACKNOWLEDGMENTS

The study was performed with the use of the All-Russia Collection of Industrial Microorganisms (Moscow, Russia) of the National Bioresource Center. The work was financially supported by the Ministry of Education and Science of the Russian Federation (Project Unique Identifier RFMEFI60717X0180).

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center, Kurchatov Institute, (NRC Kurchatov Institute, GosNIIgenetika), 117545, Moscow, Russia

    A. N. Kalinina, L. N. Borshchevskaya, T. L. Gordeeva & S. P. Sineoky

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  1. A. N. Kalinina
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  2. L. N. Borshchevskaya
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  3. T. L. Gordeeva
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  4. S. P. Sineoky
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Correspondence to A. N. Kalinina.

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Translated by V. Mittova

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Kalinina, A.N., Borshchevskaya, L.N., Gordeeva, T.L. et al. Screening and Taxonomic Characterization of Xylanase Bacterial Producers. Appl Biochem Microbiol 54, 713–716 (2018). https://doi.org/10.1134/S0003683818070037

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  • DOI: https://doi.org/10.1134/S0003683818070037

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