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The role of proteins of the outer membrane of Shewanella oneidensis MR-1 in the formation and stabilization of silver sulfide nanoparticles

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Abstract

Silver sulfide nanoparticles stable in aqueous solutions were obtained in presence of the cells of the bacterium Shewanella oneidensis MR-1 in aqueous solution containing an equimolar mixture of AgNO3 and Na2S2O3. Proteins absorbed on the surface of Ag2S nanoparticles were identified for the first time by MALDITOF/TOF. Among these proteins, multiheme cytochromes MtrC and OmcA, as well as the MtrB membrane porin, which forms a complex on the outer cell membrane, were detected. It was shown that an insoluble precipitate consisting of agglomerated Ag2S nanoparticles with a wide size distribution was formed in the absence of the cells. The role of the detected proteins in the mechanism of the formation and stabilization of the Ag2S nanoparticles in the studied system is discussed.

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Abbreviations

IR:

infrared

TEM:

transmission electron microscopy

LB medium:

Luria–Bertani medium

EPS:

extracellular polymeric substances

QD:

quantum dots

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    T. A. Voeikova, L. M. Novikova, O. A. Zhuravliova & V. G. Debabov

  2. Lomonosov Moscow State University, Moscow, 119991, Russia

    A. S. Shebanova, K. V. Shaitan & M. P. Kirpichnikov

  3. Orekhovich Institute for Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russia

    Yu. D. Ivanov, A. L. Kaysheva & V. V. Shumyantseva

  4. Research and Education Center Nanotechnologies, People’s Friendship University, Moscow, 117198, Russia

    O. A. Zhuravliova

  5. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia

    K. V. Shaitan

Authors
  1. T. A. Voeikova
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  2. A. S. Shebanova
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  3. Yu. D. Ivanov
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  4. A. L. Kaysheva
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  5. L. M. Novikova
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  6. O. A. Zhuravliova
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  7. V. V. Shumyantseva
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  8. K. V. Shaitan
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  9. M. P. Kirpichnikov
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  10. V. G. Debabov
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Corresponding author

Correspondence to T. A. Voeikova.

Additional information

Original Russian Text © T.A. Voeikova, A.S. Shebanova, Yu.D. Ivanov, A.L. Kaysheva, L.M. Novikova, O.A. Zhuravliova, V.V. Shumyantseva, K.V. Shaitan, M.P. Kirpichnikov, V.G. Debabov, 2015, published in Biotekhnologiya, 2015, No. 5, pp. 41–48.

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Voeikova, T.A., Shebanova, A.S., Ivanov, Y.D. et al. The role of proteins of the outer membrane of Shewanella oneidensis MR-1 in the formation and stabilization of silver sulfide nanoparticles. Appl Biochem Microbiol 52, 769–775 (2016). https://doi.org/10.1134/S0003683816080081

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  • DOI: https://doi.org/10.1134/S0003683816080081

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