Skip to main content
SpringerLink
Log in

Antimicrobial activity of stable silver nanoparticles of a certain size

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

Conditions for obtaining stable silver nanoparticles smaller than 10 nm were developed using a binary stabilizer polyvinylpyrrolidone/sodium dodecylsulphate in optimal ratio. Optical spectra, morphology and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal solutions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past two years were showed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Krutyakov, Yu.A., Kudrinskii, A.A., Olenin, A.Yu., and Lisichkin, G.V., Usp. Khim., 2008, vol. 77, no. 3, pp. 242–270.

    Article  Google Scholar 

  2. Marambio-Jones, C. and Hoek, E.M.V., J. Nanopart. Res., 2010, vol. 12, pp. 1531–1551.

    Article  CAS  Google Scholar 

  3. Silver, S., FEMS Microbiol. Rev., 2003, vol. 27, pp. 341–353.

    Article  PubMed  CAS  Google Scholar 

  4. Silver, S., Phung, L.T., and Silver, G., J. Ind. Microbiol. Biotechnol., 2006, vol. 33, pp. 627–634.

    Article  PubMed  CAS  Google Scholar 

  5. Clement, J.L. and Jarrett, P.S., Metal-Based Drugs, 1994, vol. 1, nos. 5–6, pp. 467–482.

    Article  PubMed  CAS  Google Scholar 

  6. Elchiguerra, J.L., Burt, J.L., Morones, J.R., Camacho-Bragado, A., Gao, X., Lara, H.H., and Yacaman, M.J., J. Nanobiotechnol., 2005, vol. 3, no. 6. doi:10.1186/1477-3155-3-6

  7. Baker, C., Pradhan, A., Pakstis, L., Pochan, D.J., and Shah, S.I., J. Nanosci. Nanotechnol., 2005, vol. 5, no. 2, pp. 244–249.

    Article  PubMed  CAS  Google Scholar 

  8. Shrivastava, S., Bera, T., Roy, A., Singh, G., and Ramachandran, P., and Das, D., Nanotecnology, 2007, vol. 18, pp. 1–9.

    Article  Google Scholar 

  9. Pal, S., Tak, Y., and Song, J.M., Appl. Environ. Microbiol., 2007, vol. 73, pp. 1712–1720.

    Article  PubMed  CAS  Google Scholar 

  10. Weir, E., Lawlor, A., Whelan, A., and Regan, F., Analyst, 2008, vol. 133, pp. 835–845.

    Article  PubMed  CAS  Google Scholar 

  11. Mukha, Yu., Eremenko, A.M., Smirnova, N.P., Korchak, G.I., Mikhienkova, A., and Chekman, I.S., in Khimiya, fizika i tekhnologiya poverkhnosti (Chemistry, Physics, and Technology of Surface), Kiev: Naukova Dumka, 2009, vol. 15, pp. 255–266.

    Google Scholar 

  12. Mikhienkova, A. and Mukha, Yu., Dovkillya Zdorov., 2011, no. 1, pp. 55–59.

  13. EN 13624:2003. Chemical Disinfectants and Antiseptics—Quantitative Suspension Test for the Evaluation of Fungicidal Activity of Chemical Disinfectants for Instruments Used in the Medical Area—Test Method and Requirements (Phase 2, Step 1).

  14. EN 13727:2003. Chemical Disinfectants and Antiseptics—Quantitative Suspension Test for the Evaluation of Bactericidal Activity of Chemical Disinfectants for Instruments Used in the Medical Area—Test Method and Requirements (Phase 2, Step 1).

  15. Morones, J.R., Elechiguerra, J.L., Camacho, A., Holt, K., Kouri, J.B., Ramirez, J.T., and Yacaman, M.J., Nanotecnology, 2005, vol. 16, no. 10, pp. 2346–2353.

    Article  CAS  Google Scholar 

  16. Henglein, A., Mulvaney, P., and Linnert, T., Faraday Discuss., 1991, vol. 92, pp. 31–44.

    Article  CAS  Google Scholar 

  17. Patakfalvi, R., Viranyi, Z., and Dekany, I., Colloid Polym. Sci., 2004, vol. 283, no. 3, pp. 299–305.

    Article  CAS  Google Scholar 

  18. Hoogsteen, W. and Fokkink, L.G.J., J. Colloid Interface Sci., 1995, vol. 175, pp. 12–26.

    Article  CAS  Google Scholar 

  19. Olofsson, G. and Wang, G., Pure Appl. Chem., 1994, vol. 66, no. 3, pp. 527–532.

    Article  CAS  Google Scholar 

  20. Sukul, D., Pal, S.K., Mandal, D., Sen, S., and Bhattacharyya, K., J. Phys. Chem. B, 2000, vol. 104, no. 26, pp. 6128–6132.

    Article  CAS  Google Scholar 

  21. Roscigno, P., Asaro, F., Pellizer, G., Ortona, O., and Paduano, L., Langmuir, 2003, vol. 19, pp. 9638–9644.

    Article  CAS  Google Scholar 

  22. Chen, Ch., Wang, L., Jiang, G., and Yu, H., Res. Adv. Mater., 2006, vol. 11, pp. 1–18.

    Article  CAS  Google Scholar 

  23. Yin, B., Ma, H., Wang, S., and Chen, S., J. Phys. Chem. B, 2003, vol. 107, pp. 8898–8904.

    Article  CAS  Google Scholar 

  24. Kora, A.J., Manjusha, R., and Arunachalam, J., Mater. Sci. Eng., 2009, vol. 29, pp. 2104–2109.

    Article  CAS  Google Scholar 

  25. Wright, J.B., Lam, K., Hansen, D., and Burrell, R.E., Am. J. Infect. Control, 1999, vol. 27, no. 4, pp. 344–349.

    Article  PubMed  CAS  Google Scholar 

  26. Wells, T.N., Scully, P., Paravicini, G., Proudfoot, A.E., and Payton, M.A., Biochemistry, 1995, vol. 34, no. 24, pp. 7896–7903.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv-164, 03164, Ukraine

    Iu. P. Mukha, A. M. Eremenko & N. P. Smirnova

  2. A. N. Marzeev Institute for Hygiene and Medical Ecology NAMS Ukraine, Kyiv-94, 02660, Ukraine

    A. I. Mikhienkova & G. I. Korchak

  3. A. V. Palladin Institute of Biochemistry NAS Ukraine, Kiev-601, 01601, Ukraine

    V. F. Gorchev & A. Yu. Chunikhin

Authors
  1. Iu. P. Mukha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Eremenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. P. Smirnova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. I. Mikhienkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. I. Korchak
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. F. Gorchev
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Yu. Chunikhin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Iu. P. Mukha.

Additional information

Original Russian Text © Iu.P. Mukha, A.M. Eremenko, N.P. Smirnova, A.I. Mikhienkova, G.I. Korchak, V.F. Gorchev, A.Yu. Chunikhin, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 2, pp. 215–223.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mukha, I.P., Eremenko, A.M., Smirnova, N.P. et al. Antimicrobial activity of stable silver nanoparticles of a certain size. Appl Biochem Microbiol 49, 199–206 (2013). https://doi.org/10.1134/S0003683813020117

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683813020117

Keywords

Search

Navigation