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The model of resting forms of micobacteria for testing of chemodrugs for latent forms of tuberculosis

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Abstract

The new model of obtaining of ovoid resting forms of Mycobacterium smegmatis, which are morphologically different from vegetative (rod-like) cells, was developed. Ovoid forms were characterized by a drastically decreased level of metabolic activity, an increased stability to heat or antibiotics treatment, and also by prolonged (more than 2 months) storage time preserving colony-forming ability. Obtained resting forms of micobacteria may be used in test-systems for checking efficiency of new medical agents against latent forms of tuberculosis and determination of role of the genes in entering dormant state.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia, 119071

    A. M. Anuchin, A. V. Goncharenko, I. V. Galon, O. I. Demidenok, Yu. K. Kudykina & A. S. Kaprelyants

  2. Moscow State University, Moscow, Russia, 119991

    M. M. Moisenovich

  3. Vinogradsky Institute of microbiology, Moscow, Russia, 117312

    A. L. Mulyukin

Authors
  1. A. M. Anuchin
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  2. A. V. Goncharenko
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  3. I. V. Galon
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  4. O. I. Demidenok
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  5. Yu. K. Kudykina
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  6. M. M. Moisenovich
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  7. A. L. Mulyukin
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  8. A. S. Kaprelyants
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Correspondence to A. M. Anuchin.

Additional information

Original Russian Text © A.M. Anuchin, A.V. Goncharenko, I.V. Galon, O.I. Demidenok, Yu.K. Kudykina, M.M. Moisenovich, A.L. Mulyukin, A.S. Kaprelyants, 2010, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2010, Vol. 46, No. 3, pp. 308–314.

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Anuchin, A.M., Goncharenko, A.V., Galon, I.V. et al. The model of resting forms of micobacteria for testing of chemodrugs for latent forms of tuberculosis. Appl Biochem Microbiol 46, 282–288 (2010). https://doi.org/10.1134/S0003683810030063

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  • DOI: https://doi.org/10.1134/S0003683810030063

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