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Enzyme immunoassay for the determination of the glycopeptide antibiotic eremomycin

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Abstract

An indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed using rabbit polyclonal antibodies against the eremomycin-glucose oxidase conjugated antigen. This technique allows the glycopeptide antibiotic eremomycin to be determined both in aqueous solutions (with a sensitivity as high as 0.1 ng/ml) and in blood plasma. The cross-reactivity of the antibodies with vancomycin was 0.4% of that for eremomycin, while teicoplanin was almost not recognized. Experiments with blood plasma samples diluted 1: 10 showed that the assay was linear over the concentration range 1–30 ng/ml and that the variation coefficient did not exceed 10%. The high sensitivity and selectivity of this test make it suitable for pharmacokinetic studies and drug monitoring analysis.

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References

  1. Gauze, G.F., Brazhnikova, M.G., Laiko, A.V., Sveshnikova, M.A., Preobrazhenskaya, T.P., and Fedorova, G.B., Antibiot. Med. Biotekhnol., 1987, vol. 32, no. 8, pp. 571–576.

    PubMed  CAS  Google Scholar 

  2. Gause, G.F., Brazhnikova, M.G., Lomakina, N.N., Berdnikova, T.F., Fedorova, G.B., Tokareva, N.L., Borisova, V.N., and Batta, G.Y., J. Antibiot., 1989, vol. 42, no. 12, pp. 1790–1797.

    PubMed  CAS  Google Scholar 

  3. Ratsional’naya antimikrobnaya farmakoterapiya. Rukovodstvo dlya praktikuyushchikh vrachei (Rational Antimicrobial Pharmacotherapy: Manual for Practicing Physicians), Yakovlev, V.P. and Yakovlev, S.V., Eds., Moscow: Litterra, 2003.

    Google Scholar 

  4. Ackerman, B.H., Berg, H.G., Strate, R.G., and Rotschafer, J.C., J. Clin. Microbiol., 1983, vol. 18, no. 4, pp. 994–995.

    PubMed  CAS  Google Scholar 

  5. Mastin, S.H., Buck, R.L., and Mueggler, P.A., Diagn. Microbiol. Infect. Dis., 1993, vol. 16, no. 1, pp. 17–24.

    Article  PubMed  CAS  Google Scholar 

  6. Lam, M.T., Le Chris X, Analyst, 2002, vol. 127, no. 12, pp. 1633–1637.

    Article  PubMed  CAS  Google Scholar 

  7. Pfaller, M.A., Krogstad, D.J., Granich, G.G., and Murray, P.R., J. Clin. Microbiol., 1984, vol. 20, no. 3, pp. 311–316.

    PubMed  CAS  Google Scholar 

  8. Corti, A., Cavenaghi, L., Giani, E., and Cassani, G., Clin. Chem., 1987, vol. 33, no. 9, pp. 1615–1618.

    PubMed  CAS  Google Scholar 

  9. Burkin, A.A., Kononenko, G.P., and Soboleva, N.A., Prikl. Biokhim. Mikrobiol., 2000, vol. 36, no. 4, pp. 428–432.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Mechnikov Research Institute for Vaccines and Sera, Russian Academy of Medical Sciences, RAMS, Moscow, 105064, Russia

    M. A. Burkin & A. A. Burkin

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  1. M. A. Burkin
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  2. A. A. Burkin
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Correspondence to M. A. Burkin.

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Original Russian Text © M.A. Burkin, A.A. Burkin, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 2, pp. 232–236.

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Burkin, M.A., Burkin, A.A. Enzyme immunoassay for the determination of the glycopeptide antibiotic eremomycin. Appl Biochem Microbiol 45, 210–214 (2009). https://doi.org/10.1134/S0003683809020161

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